Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   R5020_RS12495 Genome accession   NZ_CP137500
Coordinates   2615788..2616213 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain HPA0384     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2610788..2621213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5020_RS12475 (R5020_12475) ileS 2610945..2613776 (+) 2832 WP_003094730.1 isoleucine--tRNA ligase -
  R5020_RS12480 (R5020_12480) lspA 2613769..2614278 (+) 510 WP_003094728.1 signal peptidase II -
  R5020_RS12485 (R5020_12485) fkpB 2614271..2614711 (+) 441 WP_003094726.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  R5020_RS12490 (R5020_12490) ispH 2614797..2615741 (+) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  R5020_RS12495 (R5020_12495) comF 2615788..2616213 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  R5020_RS12500 (R5020_12500) pilY2 2616210..2616557 (-) 348 WP_003094713.1 type 4a fimbrial biogenesis protein PilY2 -
  R5020_RS12505 (R5020_12505) pilY1 2616559..2620050 (-) 3492 WP_003094704.1 type 4a pilus biogenesis protein PilY1 -
  R5020_RS12510 (R5020_12510) pilX 2620062..2620649 (-) 588 WP_003094700.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=821267 R5020_RS12495 WP_003094721.1 2615788..2616213(-) (comF) [Pseudomonas aeruginosa strain HPA0384]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=821267 R5020_RS12495 WP_003094721.1 2615788..2616213(-) (comF) [Pseudomonas aeruginosa strain HPA0384]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383