Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   RZV12_RS24135 Genome accession   NZ_CP136552
Coordinates   4880372..4881136 (+) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli O104:H4 strain rpoS_ATGtoATA     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4875372..4886136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZV12_RS24115 acpT 4876326..4876913 (+) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -
  RZV12_RS24120 nikA 4877024..4878598 (+) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  RZV12_RS24125 nikB 4878598..4879542 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  RZV12_RS24130 nikC 4879539..4880372 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  RZV12_RS24135 amiE 4880372..4881136 (+) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  RZV12_RS24140 nikE 4881133..4881939 (+) 807 WP_000173630.1 nickel import ATP-binding protein NikE -
  RZV12_RS24145 nikR 4881945..4882346 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  RZV12_RS24150 - 4882466..4882825 (-) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  RZV12_RS24155 - 4882822..4883052 (-) 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin -
  RZV12_RS24160 yhhJ 4883156..4884280 (-) 1125 WP_001216257.1 ABC transporter permease -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=815079 RZV12_RS24135 WP_001136229.1 4880372..4881136(+) (amiE) [Escherichia coli O104:H4 strain rpoS_ATGtoATA]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=815079 RZV12_RS24135 WP_001136229.1 4880372..4881136(+) (amiE) [Escherichia coli O104:H4 strain rpoS_ATGtoATA]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398