Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   KI220_RS17775 Genome accession   NZ_AP023088
Coordinates   3593177..3594211 (-) Length   344 a.a.
NCBI ID   WP_039072847.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain RSC-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3588177..3599211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI220_RS17725 (RSC1_03596) - 3588247..3589041 (-) 795 WP_020452167.1 TasA family protein -
  KI220_RS17730 (RSC1_03597) - 3589114..3589698 (-) 585 WP_020452168.1 signal peptidase I -
  KI220_RS17735 (RSC1_03598) tapA 3589695..3590423 (-) 729 WP_020452169.1 amyloid fiber anchoring/assembly protein TapA -
  KI220_RS17740 (RSC1_03599) - 3590701..3591021 (+) 321 WP_023855188.1 YqzG/YhdC family protein -
  KI220_RS17745 (RSC1_03600) - 3591051..3591233 (-) 183 WP_020452171.1 YqzE family protein -
  KI220_RS17750 (RSC1_03601) comGG 3591322..3591687 (-) 366 WP_025811163.1 competence type IV pilus minor pilin ComGG -
  KI220_RS17755 (RSC1_03602) comGF 3591699..3592187 (-) 489 WP_224067282.1 competence type IV pilus minor pilin ComGF -
  KI220_RS17760 (RSC1_03603) comGE 3592096..3592443 (-) 348 WP_023855191.1 competence type IV pilus minor pilin ComGE -
  KI220_RS17765 (RSC1_03604) comGD 3592427..3592864 (-) 438 WP_039072845.1 competence type IV pilus minor pilin ComGD -
  KI220_RS17770 (RSC1_03605) comGC 3592870..3593163 (-) 294 WP_039072846.1 competence type IV pilus major pilin ComGC Machinery gene
  KI220_RS17775 (RSC1_03606) comGB 3593177..3594211 (-) 1035 WP_039072847.1 competence type IV pilus assembly protein ComGB Machinery gene
  KI220_RS17780 (RSC1_03607) comGA 3594201..3595265 (-) 1065 WP_039072848.1 competence type IV pilus ATPase ComGA Machinery gene
  KI220_RS17785 (RSC1_03608) - 3595423..3596262 (-) 840 WP_039072849.1 STAS domain-containing protein -
  KI220_RS17795 (RSC1_03609) - 3596504..3596881 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  KI220_RS17800 (RSC1_03610) - 3597092..3597337 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  KI220_RS17805 (RSC1_03611) - 3597373..3598011 (-) 639 WP_025811156.1 MBL fold metallo-hydrolase -
  KI220_RS17810 (RSC1_03612) - 3598167..3598340 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  KI220_RS17815 (RSC1_03613) - 3598401..3598715 (-) 315 WP_020452182.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39818.13 Da        Isoelectric Point: 9.7341

>NTDB_id=81316 KI220_RS17775 WP_039072847.1 3593177..3594211(-) (comGB) [Bacillus paralicheniformis strain RSC-1]
MKIKKKWPVREQADILKKLGDMMKSGYTILDALSMLKLQLNERRRADLTFAMEKLTEGRPVFQVLEMISFHKDAVTIVYF
AEQHGNLPYAFRQSGELLHRKIAQTEKLKKAARYPLFLVLTVCVIISIMKSAIIPQFSAIYESMNVETSFATTLIFSVFD
HFYLFIAGMMLIAAALSLYYLCSFRHKPPEDKMTFLIRIPLLGQTFKLFNSYFLSLQLSNLLQAGLSVYDSLKAFESQPF
LTFHKNEAKRLIERLKQGESLEQMLAGHPFYENDLAKAVAHGQLNGHLYRELYSYSQFLIERLERKAEKWTGLIQPLIYG
LTAAMILILYLSMLLPMYQMMNQL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=81316 KI220_RS17775 WP_039072847.1 3593177..3594211(-) (comGB) [Bacillus paralicheniformis strain RSC-1]
ATGAAGATTAAAAAGAAGTGGCCCGTCCGGGAACAGGCGGACATTCTGAAAAAACTCGGAGACATGATGAAGAGCGGATA
TACGATTTTGGATGCTTTAAGTATGCTGAAACTGCAATTGAATGAAAGGCGAAGAGCAGATCTGACATTTGCGATGGAGA
AGCTGACAGAAGGGCGACCTGTGTTTCAAGTGCTTGAAATGATTTCATTTCATAAGGATGCTGTTACCATTGTTTACTTT
GCCGAACAGCACGGGAATTTGCCATATGCCTTTCGGCAGAGCGGAGAGCTGCTTCACCGCAAGATCGCTCAAACCGAAAA
ACTAAAGAAAGCCGCCAGATATCCCTTGTTTTTAGTTTTAACGGTCTGCGTCATTATCTCTATTATGAAATCGGCAATCA
TCCCGCAATTTTCCGCGATCTATGAATCAATGAACGTTGAAACGTCTTTTGCAACGACCCTCATCTTTTCGGTTTTTGAT
CATTTTTATCTTTTTATCGCAGGAATGATGTTGATTGCCGCCGCTTTATCCCTCTATTATCTGTGTTCATTTCGCCATAA
ACCTCCAGAAGACAAAATGACCTTTCTCATTAGAATTCCCTTGCTGGGCCAAACCTTCAAACTATTCAACAGTTATTTTT
TATCGCTCCAATTGAGCAATCTTTTGCAAGCTGGTCTGTCTGTATATGACAGTTTAAAAGCATTTGAAAGCCAGCCGTTT
TTAACATTTCATAAAAATGAAGCGAAGCGCCTGATCGAGCGGCTGAAACAAGGGGAGAGTCTGGAGCAGATGCTGGCCGG
GCATCCGTTTTATGAAAATGATCTGGCGAAGGCTGTTGCTCACGGCCAATTAAACGGACACTTATACAGAGAGCTGTATT
CATACAGTCAATTTTTGATTGAACGGCTTGAACGGAAAGCAGAAAAATGGACAGGTTTGATCCAGCCATTGATTTACGGG
CTAACAGCGGCGATGATATTAATTCTTTATTTGTCTATGCTTTTGCCAATGTATCAAATGATGAACCAGTTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(216-336)

Type II secretion system (T2SS), protein F (T2SSF)

(16-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

57.276

93.895

0.538

Multiple sequence alignment