Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   RUW23_RS18280 Genome accession   NZ_CP136014
Coordinates   3905013..3905438 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain R18-20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3900013..3910438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RUW23_RS18260 (RUW23_18260) ileS 3900170..3903001 (+) 2832 WP_023103515.1 isoleucine--tRNA ligase -
  RUW23_RS18265 (RUW23_18265) lspA 3902994..3903503 (+) 510 WP_003110420.1 signal peptidase II -
  RUW23_RS18270 (RUW23_18270) fkpB 3903496..3903936 (+) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  RUW23_RS18275 (RUW23_18275) ispH 3904022..3904966 (+) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  RUW23_RS18280 (RUW23_18280) comF 3905013..3905438 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  RUW23_RS18285 (RUW23_18285) pilY2 3905435..3905782 (-) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  RUW23_RS18290 (RUW23_18290) pilY1 3905784..3909275 (-) 3492 WP_003102607.1 type 4a pilus biogenesis protein PilY1 -
  RUW23_RS18295 (RUW23_18295) pilX 3909287..3909874 (-) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=812680 RUW23_RS18280 WP_003094721.1 3905013..3905438(-) (comF) [Pseudomonas aeruginosa strain R18-20]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=812680 RUW23_RS18280 WP_003094721.1 3905013..3905438(-) (comF) [Pseudomonas aeruginosa strain R18-20]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAATCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383