Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   RS158_RS21680 Genome accession   NZ_CP135712
Coordinates   4467933..4468697 (+) Length   254 a.a.
NCBI ID   WP_001136211.1    Uniprot ID   -
Organism   Escherichia coli strain TUM1586     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4462933..4473697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS158_RS21660 (RS158_21660) acpT 4463887..4464474 (+) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -
  RS158_RS21665 (RS158_21665) nikA 4464585..4466159 (+) 1575 WP_000493128.1 nickel ABC transporter substrate-binding protein -
  RS158_RS21670 (RS158_21670) nikB 4466159..4467103 (+) 945 WP_000947080.1 nickel ABC transporter permease subunit NikB -
  RS158_RS21675 (RS158_21675) nikC 4467100..4467933 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  RS158_RS21680 (RS158_21680) amiE 4467933..4468697 (+) 765 WP_001136211.1 nickel import ATP-binding protein NikD Regulator
  RS158_RS21685 (RS158_21685) nikE 4468694..4469500 (+) 807 WP_000173687.1 nickel import ATP-binding protein NikE -
  RS158_RS21690 (RS158_21690) nikR 4469506..4469907 (+) 402 WP_001189000.1 nickel-responsive transcriptional regulator NikR -
  RS158_RS21695 (RS158_21695) - 4470106..4470852 (+) 747 WP_001329803.1 GntR family transcriptional regulator -
  RS158_RS21700 (RS158_21700) - 4470877..4471350 (+) 474 WP_012311717.1 PTS sugar transporter subunit IIA -
  RS158_RS21705 (RS158_21705) - 4471347..4471628 (+) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  RS158_RS21710 (RS158_21710) - 4471702..4473060 (+) 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.45 Da        Isoelectric Point: 6.9016

>NTDB_id=812182 RS158_RS21680 WP_001136211.1 4467933..4468697(+) (amiE) [Escherichia coli strain TUM1586]
MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=812182 RS158_RS21680 WP_001136211.1 4467933..4468697(+) (amiE) [Escherichia coli strain TUM1586]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTTAA
ACGCGGGCGTGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCACTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGTGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTTCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCACGCGTGC
TGAAGCTGTACCCGTTTGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGTTGTGTGAATCGCCG
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATTCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAATGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398