Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RS158_RS20980 Genome accession   NZ_CP135712
Coordinates   4320054..4320521 (+) Length   155 a.a.
NCBI ID   WP_000340159.1    Uniprot ID   -
Organism   Escherichia coli strain TUM1586     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4315054..4325521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS158_RS20935 (RS158_20935) rplP 4315164..4315574 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  RS158_RS20940 (RS158_20940) rpsC 4315587..4316288 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  RS158_RS20945 (RS158_20945) rplV 4316306..4316638 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  RS158_RS20950 (RS158_20950) rpsS 4316653..4316931 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  RS158_RS20955 (RS158_20955) rplB 4316948..4317769 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  RS158_RS20960 (RS158_20960) rplW 4317787..4318089 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  RS158_RS20965 (RS158_20965) rplD 4318086..4318691 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  RS158_RS20970 (RS158_20970) rplC 4318702..4319331 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  RS158_RS20975 (RS158_20975) rpsJ 4319364..4319675 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  RS158_RS20980 (RS158_20980) pilD 4320054..4320521 (+) 468 WP_000340159.1 prepilin peptidase Machinery gene
  RS158_RS20985 (RS158_20985) bfr 4320518..4320994 (-) 477 WP_000675515.1 bacterioferritin -
  RS158_RS20990 (RS158_20990) bfd 4321067..4321261 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  RS158_RS20995 (RS158_20995) tuf 4321444..4322628 (-) 1185 WP_000031783.1 elongation factor Tu -
  RS158_RS21000 (RS158_21000) fusA 4322699..4324813 (-) 2115 WP_000124700.1 elongation factor G -
  RS158_RS21005 (RS158_21005) rpsG 4324910..4325380 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17034.17 Da        Isoelectric Point: 8.1255

>NTDB_id=812178 RS158_RS20980 WP_000340159.1 4320054..4320521(+) (pilD) [Escherichia coli strain TUM1586]
MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=812178 RS158_RS20980 WP_000340159.1 4320054..4320521(+) (pilD) [Escherichia coli strain TUM1586]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACATGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGCTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.882

81.935

0.368