Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   RS163_RS01215 Genome accession   NZ_CP135688
Coordinates   269938..270702 (-) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain TUM2820     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 264938..275702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS163_RS01205 (RS163_01205) nikR 268728..269129 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  RS163_RS01210 (RS163_01210) nikE 269135..269941 (-) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  RS163_RS01215 (RS163_01215) amiE 269938..270702 (-) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  RS163_RS01220 (RS163_01220) nikC 270702..271535 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  RS163_RS01225 (RS163_01225) nikB 271532..272476 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  RS163_RS01230 (RS163_01230) nikA 272476..274050 (-) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  RS163_RS01235 (RS163_01235) acpT 274161..274748 (-) 588 WP_000285791.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=811697 RS163_RS01215 WP_001136236.1 269938..270702(-) (amiE) [Escherichia coli strain TUM2820]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=811697 RS163_RS01215 WP_001136236.1 269938..270702(-) (amiE) [Escherichia coli strain TUM2820]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398