Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   RQ468_RS25005 Genome accession   NZ_CP135175
Coordinates   5364237..5364662 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain PARM_Z1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5359237..5369662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RQ468_RS24990 (RQ468_24990) pilX 5359801..5360388 (+) 588 WP_003102605.1 type 4a pilus minor pilin PilX -
  RQ468_RS24995 (RQ468_24995) pilY1 5360400..5363891 (+) 3492 WP_003102607.1 type 4a pilus biogenesis protein PilY1 -
  RQ468_RS25000 (RQ468_25000) pilY2 5363893..5364240 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  RQ468_RS25005 (RQ468_25005) comF 5364237..5364662 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  RQ468_RS25010 (RQ468_25010) ispH 5364709..5365653 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  RQ468_RS25015 (RQ468_25015) fkpB 5365739..5366179 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  RQ468_RS25020 (RQ468_25020) lspA 5366172..5366681 (-) 510 WP_003102615.1 signal peptidase II -
  RQ468_RS25025 (RQ468_25025) ileS 5366674..5369505 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=810490 RQ468_RS25005 WP_003094721.1 5364237..5364662(+) (comF) [Pseudomonas aeruginosa strain PARM_Z1]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=810490 RQ468_RS25005 WP_003094721.1 5364237..5364662(+) (comF) [Pseudomonas aeruginosa strain PARM_Z1]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383