Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ROT04_RS28290 Genome accession   NZ_CP135174
Coordinates   6067506..6069290 (+) Length   594 a.a.
NCBI ID   WP_058354836.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PARM_L1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6062506..6074290
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ROT04_RS28270 (ROT04_28270) argE 6062744..6063898 (-) 1155 WP_003457770.1 acetylornithine deacetylase -
  ROT04_RS28275 (ROT04_28275) - 6063998..6065266 (-) 1269 WP_058354834.1 inorganic phosphate transporter -
  ROT04_RS28280 (ROT04_28280) - 6065313..6065990 (-) 678 WP_003096272.1 TIGR00153 family protein -
  ROT04_RS28285 (ROT04_28285) - 6066104..6067468 (+) 1365 WP_058354835.1 inorganic triphosphatase -
  ROT04_RS28290 (ROT04_28290) pilB 6067506..6069290 (+) 1785 WP_058354836.1 GspE/PulE family protein Machinery gene
  ROT04_RS28295 (ROT04_28295) - 6069512..6069964 (+) 453 WP_003109994.1 NfeD family protein -
  ROT04_RS28300 (ROT04_28300) - 6070096..6070425 (+) 330 WP_003096280.1 DUF2388 domain-containing protein -
  ROT04_RS28305 (ROT04_28305) gcvP 6070460..6073336 (-) 2877 WP_058354837.1 aminomethyl-transferring glycine dehydrogenase -
  ROT04_RS28310 (ROT04_28310) gcvH 6073510..6073899 (-) 390 WP_003107397.1 glycine cleavage system protein GcvH -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 65997.61 Da        Isoelectric Point: 6.5096

>NTDB_id=810404 ROT04_RS28290 WP_058354836.1 6067506..6069290(+) (pilB) [Pseudomonas aeruginosa strain PARM_L1]
MSVFATAAQDRLLDLGDLLRELVAQGRVGQEQAEQCLAIRRSAVRNQQHPLEFLAAQQVEDLKRPGRKLDLETLTQWLAE
YAGQPYLRIDPLKIDVAAVTPLMSYAFAQRHGILAVALSPEEVTIASAQPFVQGWESNLVHVLKRPIKRVVANPADIQRY
TVEFFRLARSVSGATSPDQKISGVGNFEQLLNLGAADQEPDANDAHIVTIVDWLFQYAFQQRASDIHIEPRREQGTVRFR
IDGVLHNVYQFPPQVTMAVVSRLKSLGRMNVAEKRKPQDGRVKTKTPDGAEVELRLSTLPTAFGEKMVMRIFDPEVLLKS
FDQLGFSADDLRRWQSMTSQPNGIILVTGPTGSGKTTTLYTTLKQLATDEVNVCTIEDPIEMIEPSFNQMQVQHNIDLTF
ASGVRALLRQDPDIIMVGEIRDLETAEMAIQAALTGHLVLSTLHTNDSPTAITRLLELGVPYYLIRATVLGVMAQRLVRT
LCPHCKAPMQLADSDWHELTRPWSAPPPSGAHQAVGCLECRDTGYRGRSGVYEIMLLSDSLKELITADTDLVALRRQAYK
EGTRSLRLSGAQKVAAGISTLEEVLRVTPQSEQK

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=810404 ROT04_RS28290 WP_058354836.1 6067506..6069290(+) (pilB) [Pseudomonas aeruginosa strain PARM_L1]
ATGTCCGTGTTCGCCACCGCTGCCCAGGACCGCCTGCTGGACCTCGGCGACCTGCTCCGCGAGCTGGTCGCCCAGGGCCG
GGTCGGGCAGGAGCAGGCCGAACAGTGCCTGGCGATCCGCCGCAGCGCGGTACGCAACCAGCAGCATCCGCTGGAGTTCC
TCGCCGCTCAGCAGGTCGAGGATCTCAAGCGCCCCGGACGCAAGCTCGACCTGGAGACCCTCACCCAGTGGCTGGCCGAG
TACGCCGGCCAGCCCTACCTGCGCATCGACCCGCTGAAGATCGACGTCGCCGCGGTGACTCCGCTGATGTCCTACGCCTT
CGCCCAGCGCCATGGCATCCTCGCCGTCGCGCTGAGTCCGGAGGAAGTCACCATCGCCAGCGCCCAGCCCTTCGTACAGG
GCTGGGAAAGCAACCTGGTGCATGTGCTCAAGCGACCGATCAAGCGGGTGGTGGCGAACCCGGCGGACATCCAGCGCTAC
ACCGTCGAGTTCTTCCGCCTGGCCCGCTCGGTCAGCGGCGCCACCTCGCCGGACCAGAAGATCAGCGGCGTCGGCAACTT
CGAGCAGTTGCTCAACCTCGGCGCCGCCGACCAGGAGCCGGACGCCAACGACGCGCACATCGTCACCATCGTCGACTGGC
TGTTCCAGTACGCCTTCCAGCAGCGCGCCAGCGACATCCACATCGAGCCGCGCCGCGAGCAGGGCACCGTGCGCTTCCGT
ATCGACGGCGTGCTGCACAACGTCTACCAGTTCCCGCCGCAGGTGACCATGGCGGTGGTCAGCCGCCTGAAGTCGCTGGG
ACGGATGAACGTGGCGGAGAAACGCAAGCCGCAGGACGGCCGGGTCAAGACCAAGACCCCGGACGGCGCCGAGGTGGAGC
TGCGCCTGTCGACCCTGCCCACCGCCTTCGGCGAGAAGATGGTGATGCGCATCTTCGACCCCGAGGTGCTGCTCAAGAGC
TTCGACCAGCTCGGCTTCTCCGCCGACGACCTGCGCCGCTGGCAGAGCATGACCAGCCAGCCCAACGGCATCATCCTGGT
CACCGGCCCCACCGGCTCGGGCAAGACCACCACGCTCTACACCACGCTCAAGCAACTGGCCACCGATGAGGTGAACGTCT
GCACCATCGAGGACCCGATCGAGATGATCGAGCCCTCGTTCAACCAGATGCAGGTCCAGCACAACATCGACCTGACCTTC
GCCAGCGGCGTACGCGCCCTGCTGCGGCAGGACCCGGACATCATCATGGTCGGCGAGATCCGCGACCTGGAGACCGCCGA
GATGGCGATCCAGGCGGCGCTCACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACGACTCGCCCACGGCGATCACCC
GCCTGCTCGAACTGGGCGTGCCCTACTACCTGATCCGCGCCACGGTGCTAGGGGTCATGGCCCAGCGCCTGGTGCGCACA
CTCTGCCCGCACTGCAAGGCGCCGATGCAACTGGCGGACAGCGACTGGCACGAACTGACCCGCCCCTGGAGCGCCCCGCC
GCCCAGCGGCGCGCACCAGGCCGTGGGTTGCCTGGAGTGCCGCGACACCGGCTATCGCGGGCGCTCCGGCGTCTATGAAA
TCATGCTGCTCAGCGATAGCCTGAAAGAACTGATCACCGCCGACACCGACCTGGTCGCCCTGCGCCGCCAGGCGTACAAG
GAAGGCACCCGCAGCCTGCGCCTGTCCGGCGCGCAGAAGGTCGCCGCGGGTATCAGTACGCTGGAGGAAGTACTGCGGGT
GACCCCGCAGAGCGAACAGAAGTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(216-480)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(69-167)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.682

90.067

0.375