Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   ROT04_RS24770 Genome accession   NZ_CP135174
Coordinates   5287348..5287773 (+) Length   141 a.a.
NCBI ID   WP_023107845.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PARM_L1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5282348..5292773
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ROT04_RS24755 (ROT04_24755) pilX 5282918..5283505 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  ROT04_RS24760 (ROT04_24760) pilY1 5283517..5287002 (+) 3486 WP_058355286.1 type 4a pilus biogenesis protein PilY1 -
  ROT04_RS24765 (ROT04_24765) pilY2 5287004..5287351 (+) 348 WP_021264112.1 type 4a fimbrial biogenesis protein PilY2 -
  ROT04_RS24770 (ROT04_24770) comF 5287348..5287773 (+) 426 WP_023107845.1 type 4a pilus minor pilin PilE Machinery gene
  ROT04_RS24775 (ROT04_24775) ispH 5287820..5288764 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  ROT04_RS24780 (ROT04_24780) fkpB 5288850..5289290 (-) 441 WP_003094726.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  ROT04_RS24785 (ROT04_24785) lspA 5289283..5289792 (-) 510 WP_003094728.1 signal peptidase II -
  ROT04_RS24790 (ROT04_24790) ileS 5289785..5292616 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15309.33 Da        Isoelectric Point: 10.0198

>NTDB_id=810390 ROT04_RS24770 WP_023107845.1 5287348..5287773(+) (comF) [Pseudomonas aeruginosa strain PARM_L1]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVTKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=810390 ROT04_RS24770 WP_023107845.1 5287348..5287773(+) (comF) [Pseudomonas aeruginosa strain PARM_L1]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGACCAAACTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

41.406

90.78

0.376