Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   RPM46_RS09240 Genome accession   NZ_CP135096
Coordinates   2021044..2021469 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain Paer3554     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2016044..2026469
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RPM46_RS09220 (RPM46_09220) ileS 2016201..2019032 (+) 2832 WP_003102617.1 isoleucine--tRNA ligase -
  RPM46_RS09225 (RPM46_09225) lspA 2019025..2019534 (+) 510 WP_003102615.1 signal peptidase II -
  RPM46_RS09230 (RPM46_09230) fkpB 2019527..2019967 (+) 441 WP_003161775.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  RPM46_RS09235 (RPM46_09235) ispH 2020053..2020997 (+) 945 WP_003134939.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  RPM46_RS09240 (RPM46_09240) comF 2021044..2021469 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  RPM46_RS09245 (RPM46_09245) pilY2 2021466..2021813 (-) 348 WP_003161774.1 type 4a fimbrial biogenesis protein PilY2 -
  RPM46_RS09250 (RPM46_09250) pilY1 2021815..2025306 (-) 3492 WP_003102607.1 type 4a pilus biogenesis protein PilY1 -
  RPM46_RS09255 (RPM46_09255) pilX 2025318..2025905 (-) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=809411 RPM46_RS09240 WP_003094721.1 2021044..2021469(-) (comF) [Pseudomonas aeruginosa strain Paer3554]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=809411 RPM46_RS09240 WP_003094721.1 2021044..2021469(-) (comF) [Pseudomonas aeruginosa strain Paer3554]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAACTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACACCCACCAGCACTACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383