Detailed information NTG0807986

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P4B18_RS02075
• Genome accession: NZ_CP120705
• Coordinates: 447739..448887 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain NY11084
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 442739..453887

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P4B18_RS02050 (P4B18_02050)	-	443890..444186 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
P4B18_RS02055 (P4B18_02055)	-	444183..444776 (-)	594	WP_003084544.1	YggT family protein	-
P4B18_RS02060 (P4B18_02060)	proC	444787..445608 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
P4B18_RS02065 (P4B18_02065)	-	445620..446312 (-)	693	WP_003118803.1	YggS family pyridoxal phosphate-dependent enzyme	-
P4B18_RS02070 (P4B18_02070)	pilT	446527..447561 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
P4B18_RS02075 (P4B18_02075)	pilU	447739..448887 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
P4B18_RS02080 (P4B18_02080)	czcD	448895..449794 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
P4B18_RS02085 (P4B18_02085)	-	449949..450353 (+)	405	WP_003084560.1	NINE protein	-
P4B18_RS02090 (P4B18_02090)	-	450596..451969 (+)	1374	WP_179063077.1	pyridoxal-phosphate dependent enzyme	-
P4B18_RS02095 (P4B18_02095)	-	451966..453150 (+)	1185	WP_003117897.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=807986 P4B18_RS02075 WP_003100959.1 447739..448887(+) (pilU) [Pseudomonas aeruginosa strain NY11084]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=807986 P4B18_RS02075 WP_003100959.1 447739..448887(+) (pilU) [Pseudomonas aeruginosa strain NY11084]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382