Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   RLE12_RS27480 Genome accession   NZ_CP134695
Coordinates   5823872..5824297 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain TJM4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5818872..5829297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RLE12_RS27465 pilX 5819436..5820023 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  RLE12_RS27470 pilY1 5820035..5823526 (+) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  RLE12_RS27475 pilY2 5823528..5823875 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  RLE12_RS27480 comF 5823872..5824297 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  RLE12_RS27485 ispH 5824344..5825288 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  RLE12_RS27490 fkpB 5825374..5825814 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  RLE12_RS27495 lspA 5825807..5826316 (-) 510 WP_003102615.1 signal peptidase II -
  RLE12_RS27500 ileS 5826309..5829140 (-) 2832 WP_004352756.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=807465 RLE12_RS27480 WP_003094721.1 5823872..5824297(+) (comF) [Pseudomonas aeruginosa strain TJM4]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=807465 RLE12_RS27480 WP_003094721.1 5823872..5824297(+) (comF) [Pseudomonas aeruginosa strain TJM4]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383