Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   P5649_RS18150 Genome accession   NZ_CP120613
Coordinates   3472826..3473719 (-) Length   297 a.a.
NCBI ID   WP_003245871.1    Uniprot ID   A0AAE2SJU2
Organism   Bacillus subtilis strain DSM 23521     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3467826..3478719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5649_RS18135 (P5649_18135) xerC 3468198..3469112 (-) 915 WP_003231988.1 tyrosine recombinase XerC -
  P5649_RS18140 (P5649_18140) trmFO 3469180..3470487 (-) 1308 WP_003244725.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  P5649_RS18145 (P5649_18145) topA 3470563..3472638 (-) 2076 WP_003245599.1 type I DNA topoisomerase -
  P5649_RS18150 (P5649_18150) dprA 3472826..3473719 (-) 894 WP_003245871.1 DNA-processing protein DprA Machinery gene
  P5649_RS18155 (P5649_18155) sucD 3473780..3474682 (-) 903 WP_003238566.1 succinate--CoA ligase subunit alpha -
  P5649_RS18160 (P5649_18160) sucC 3474711..3475868 (-) 1158 WP_003244732.1 ADP-forming succinate--CoA ligase subunit beta -
  P5649_RS18165 (P5649_18165) ylqH 3476041..3476322 (-) 282 WP_003232001.1 FlhB-like flagellar biosynthesis protein -
  P5649_RS18170 (P5649_18170) - 3476319..3478049 (-) 1731 WP_003245096.1 hypothetical protein -

Sequence

Protein


Download         Length: 297 a.a.        Molecular weight: 32935.26 Da        Isoelectric Point: 9.9142

>NTDB_id=806934 P5649_RS18150 WP_003245871.1 3472826..3473719(-) (dprA) [Bacillus subtilis strain DSM 23521]
MDQAAVCLTICRINQLLSPSLLLKWWKADPSMSLTSPVLQTVTRDQIKAAALKNEIEQFYPKLPRVLAAYREQGINTIPI
SSKQYPFWLKSIYDPPAVLFAKGDMTLLSKGRKIGIVGTRNPTAYGKQVVNHLTKEICRKGWVIVSGLASGIDGMSHAAS
IKAKGRTIGVIAGGFQHIYPRENLQLADHMAKHHILLSEHPPETKPQKWHFPMRNRIISGLSEGVIVVQGKEKSGSLITA
YQALEQGREVFAVPGSLFDPYAGGPIKLIQQGAKAIWSAEDIFEELPERNVQYTEPF

Nucleotide


Download         Length: 894 bp        

>NTDB_id=806934 P5649_RS18150 WP_003245871.1 3472826..3473719(-) (dprA) [Bacillus subtilis strain DSM 23521]
TTGGATCAGGCCGCTGTCTGCCTAACGATTTGCAGAATCAATCAATTATTATCCCCATCCCTTCTATTAAAATGGTGGAA
AGCCGATCCGTCTATGTCGCTGACATCACCCGTGTTACAAACGGTTACTCGTGATCAAATAAAAGCAGCTGCATTAAAAA
ACGAAATAGAACAATTTTATCCAAAGCTCCCGCGTGTACTTGCTGCTTATCGTGAGCAAGGCATTAACACCATCCCTATT
TCTTCAAAGCAATATCCTTTCTGGCTTAAAAGCATTTATGATCCCCCTGCCGTACTGTTTGCAAAAGGTGATATGACTCT
TCTTTCGAAAGGGAGAAAAATTGGAATTGTAGGCACAAGAAATCCAACAGCTTATGGGAAACAAGTTGTCAATCATCTTA
CAAAAGAGATCTGTCGTAAAGGTTGGGTGATTGTCAGCGGACTGGCGTCTGGGATAGACGGAATGTCCCATGCTGCAAGT
ATTAAGGCGAAGGGGCGGACAATTGGCGTCATTGCAGGCGGATTTCAACACATTTATCCCCGAGAAAACCTTCAGTTAGC
AGATCACATGGCTAAACACCATATCCTGCTGTCAGAGCACCCACCTGAAACTAAACCCCAAAAATGGCATTTCCCTATGA
GAAACCGTATTATCAGCGGACTAAGTGAAGGCGTTATTGTCGTTCAGGGCAAAGAAAAAAGCGGTTCGCTGATTACTGCC
TATCAAGCATTGGAACAAGGGAGAGAGGTATTTGCCGTACCCGGTTCATTGTTTGACCCTTACGCCGGAGGTCCTATAAA
ACTGATCCAGCAGGGGGCTAAAGCCATATGGTCAGCAGAGGATATTTTCGAGGAACTTCCTGAGAGAAACGTTCAATATA
CGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(71-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  dprA Legionella pneumophila strain ERS1305867

38.462

100

0.387
  dprA Lactococcus lactis subsp. cremoris KW2

42.105

89.562

0.377