Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   P5636_RS09595 Genome accession   NZ_CP120609
Coordinates   1756914..1759256 (-) Length   780 a.a.
NCBI ID   WP_013085100.1    Uniprot ID   -
Organism   Priestia megaterium DSM 319     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1751914..1764256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5636_RS09570 (P5636_09570) - 1752307..1752483 (-) 177 WP_013085097.1 hypothetical protein -
  P5636_RS09575 (P5636_09575) - 1752650..1753534 (+) 885 WP_258468995.1 N-acetylmuramoyl-L-alanine amidase -
  P5636_RS09580 (P5636_09580) dtd 1753525..1753986 (-) 462 WP_013085099.1 D-aminoacyl-tRNA deacylase -
  P5636_RS09585 (P5636_09585) - 1754002..1756200 (-) 2199 WP_013059308.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  P5636_RS09590 (P5636_09590) - 1756386..1756898 (-) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  P5636_RS09595 (P5636_09595) recJ 1756914..1759256 (-) 2343 WP_013085100.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  P5636_RS09600 (P5636_09600) secDF 1759397..1761634 (-) 2238 WP_013085101.1 protein translocase subunit SecDF -
  P5636_RS09605 (P5636_09605) - 1761772..1762065 (-) 294 WP_013085102.1 post-transcriptional regulator -
  P5636_RS09610 (P5636_09610) spoVB 1762190..1763740 (+) 1551 WP_042453185.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87873.47 Da        Isoelectric Point: 4.6963

>NTDB_id=806759 P5636_RS09595 WP_013085100.1 1756914..1759256(-) (recJ) [Priestia megaterium DSM 319]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADVDFYIPNRFTEGYGPNKAAFDKISAGGYQLVITVDTGISAVEETAYAREL
NFDLIITDHHEPGPVLPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEINEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDALNKV
RQQIVNDITEEAVEFVENMYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSIDSENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRTRLNEQASNVLTEQDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSDVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAVDVKRFFEQVFTDQMKVVAFHDSTIDKYKSLLPMEHVVLLQNEEQAKACMFAGEQIVLLDLPPLQ
KWLEALFVQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSAKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=806759 P5636_RS09595 WP_013085100.1 1756914..1759256(-) (recJ) [Priestia megaterium DSM 319]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCGTTTTTATTGCACGATATGGACAAAGCAGTAGCTCGTATCCAGCAAGCAATTGATCATAAT
GAAAAAATTATGATTTATGGAGATTACGATGCTGACGGCGTCAGCAGCACTTCTGTTATGTTATCTGCTTTACAGCAGCT
TGGCGCTGATGTTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCGGCATTTGATAAAATAA
GCGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACTGCGTATGCACGTGAACTC
AACTTTGATTTAATTATTACGGATCACCACGAGCCGGGGCCGGTTTTACCAGATGCTTATGCAATTATTCATCCAAAGCT
GCCGGGAAGTACATATCCATTTAAAGAACTGGCAGGTGTAGGAGTCGCTTTTAAACTCGCTCATGCACTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTAATTGGGACGATTGCTGACTTGGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGACTATCTCAAGCTCACGAAGCGCACCGGAATTGAAGCGTTGCTGAGCGTATGCGGAGTAAAACG
TGAAGAAATTAATGAGGATACAATCGGATTTGCTATGGCTCCTCGCATTAATGCAGTAGGACGTCTTCAAGATGCTGATC
CAGCTGTTCACTTGCTTATGACAACAAATGCTGAAGAAGCAAAAGGTTTAGCTAAAGAAATTGATGCGTTAAACAAGGTA
AGGCAGCAAATCGTAAACGATATTACGGAAGAAGCCGTTGAATTTGTTGAAAATATGTATCCTCCGGATGAAAACAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGCAGGTGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAGTTCTACAGAC
CAACAATTGTACTAAGTATTGACTCTGAAAACCGTATTGCCAAAGGTTCAGCAAGAAGTATCGAAGGCTTTGACTTATTC
GCGAACTTATCTAAATGCCGTGACATTTTGCCGCATTTTGGAGGGCATCCAATGGCAGCCGGCATGACGTTAAACAGCGA
TGATGTAGAAGAGCTGCGCACACGTCTTAATGAACAAGCAAGCAACGTGTTAACCGAACAGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGCACAACAGAGGAAATTACGCTGGAAAACATTGAACAAATGAGTGCGCTTGCTCCATTTGGTATG
CATAACCCGAAGCCAAAAGTTCTCATTAAAGATGTACATACAACATCTATGCGGAAAATTGGCGCTAATAAAAATCATTT
AAAACTGGTGTTTGAAGACAGCGATGTATCGGTTGATGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAGGTATCATTAGTAGGTGAACTATCTATTAATGAGTGGAATAATATGCGCAAACCGCAGCTGATGGTAGAA
GATATCTCTGTAGATGAGTGGCAGATGTTTGATTATCGCAGTGCGGTTGATGTCAAACGTTTCTTTGAGCAGGTATTCAC
GGATCAAATGAAAGTTGTTGCTTTTCACGATTCAACAATTGATAAATACAAGTCGCTGCTGCCGATGGAGCATGTTGTTC
TTCTTCAAAATGAAGAACAGGCAAAAGCATGTATGTTTGCAGGGGAACAGATCGTTTTATTAGATTTGCCTCCTTTACAG
AAGTGGCTTGAAGCTTTGTTTGTCCAAGGTATTCCTTCTAGAATATACGCTATTTTCTTAACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCACTTTAAATGGTTTTACGGATTTTTAGCGAAAAAGGGTCCTTTTGATTTAAAACGCT
ACAAAGAAGATTTAGCGATTCATAAGGGCTGGTCGAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTGCGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(305-439)

DHH family (DHH)

(82-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.385

100

0.554