Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   P5636_RS05775 Genome accession   NZ_CP120609
Coordinates   1026402..1026776 (+) Length   124 a.a.
NCBI ID   WP_258469325.1    Uniprot ID   -
Organism   Priestia megaterium DSM 319     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1021402..1031776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5636_RS05755 (P5636_05755) - 1021895..1022566 (-) 672 WP_013084544.1 response regulator transcription factor -
  P5636_RS05760 (P5636_05760) - 1022892..1023710 (+) 819 WP_013084545.1 phytoene/squalene synthase family protein -
  P5636_RS05765 (P5636_05765) - 1023746..1025695 (-) 1950 WP_013084546.1 FUSC family protein -
  P5636_RS05770 (P5636_05770) - 1025852..1026112 (-) 261 WP_042453142.1 metal-sensitive transcriptional regulator -
  P5636_RS05775 (P5636_05775) nucA/comI 1026402..1026776 (+) 375 WP_258469325.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  P5636_RS05780 (P5636_05780) comJ 1026812..1027219 (+) 408 WP_013084549.1 competence protein ComJ -
  P5636_RS05785 (P5636_05785) helD 1027248..1029566 (-) 2319 WP_013084550.1 RNA polymerase recycling motor HelD -
  P5636_RS05790 (P5636_05790) - 1029811..1029936 (-) 126 WP_016765178.1 hypothetical protein -
  P5636_RS05795 (P5636_05795) - 1030077..1030592 (-) 516 WP_013084551.1 enoyl-CoA hydratase/isomerase family protein -
  P5636_RS05800 (P5636_05800) - 1030835..1031203 (+) 369 WP_013084552.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 124 a.a.        Molecular weight: 13842.11 Da        Isoelectric Point: 4.2132

>NTDB_id=806749 P5636_RS05775 WP_258469325.1 1026402..1026776(+) (nucA/comI) [Priestia megaterium DSM 319]
MGHAAFLYPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEANREESLKGYLTKTGYDRDEWPMA
MCEEGGAGADIRYISPSDNREAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 375 bp        

>NTDB_id=806749 P5636_RS05775 WP_258469325.1 1026402..1026776(+) (nucA/comI) [Priestia megaterium DSM 319]
ATGGGACATGCAGCTTTTTTATATCCTGAAAAAACGGAAGCTGAAGTAAATTATGATGCGACACTTTATTTTCCGTTAGA
CCGATATCCTGAAACGGGTGATCATATTCGTGACGCGATTGCCGCTGGGCACTCTTCCGTTTGCACAATTGATCGCGACG
GTGCTGAAGCGAACCGAGAAGAATCCCTGAAAGGATATCTGACAAAAACAGGTTATGACCGTGATGAATGGCCAATGGCT
ATGTGCGAAGAAGGCGGAGCAGGAGCTGACATTCGCTATATTTCGCCTTCTGATAACCGTGAAGCCGGCTCATGGGTTGG
AAATCAGTTAGAACAGTATCCAGATGGTACACGCGTACAATTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(30-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

64.706

95.968

0.621