Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   RJ644_RS01260 Genome accession   NZ_CP134376
Coordinates   271259..272023 (-) Length   254 a.a.
NCBI ID   WP_001136235.1    Uniprot ID   -
Organism   Escherichia coli strain TUM12355     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 266259..277023
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ644_RS01235 (RJ644_01235) yhhJ 268101..269225 (+) 1125 WP_129924999.1 ABC transporter permease -
  RJ644_RS01240 (RJ644_01240) - 269298..269573 (+) 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin -
  RJ644_RS01245 (RJ644_01245) - 269570..269929 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  RJ644_RS01250 (RJ644_01250) nikR 270049..270450 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  RJ644_RS01255 (RJ644_01255) nikE 270456..271262 (-) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  RJ644_RS01260 (RJ644_01260) amiE 271259..272023 (-) 765 WP_001136235.1 nickel import ATP-binding protein NikD Regulator
  RJ644_RS01265 (RJ644_01265) nikC 272023..272856 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  RJ644_RS01270 (RJ644_01270) nikB 272853..273797 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  RJ644_RS01275 (RJ644_01275) nikA 273797..275371 (-) 1575 WP_000953353.1 nickel ABC transporter substrate-binding protein -
  RJ644_RS01280 (RJ644_01280) acpT 275482..276069 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26863.51 Da        Isoelectric Point: 6.5992

>NTDB_id=803147 RJ644_RS01260 WP_001136235.1 271259..272023(-) (amiE) [Escherichia coli strain TUM12355]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVEMLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=803147 RJ644_RS01260 WP_001136235.1 271259..272023(-) (amiE) [Escherichia coli strain TUM12355]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAATGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398