Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   RJ642_RS01350 Genome accession   NZ_CP134370
Coordinates   295645..296409 (-) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain TUM12357     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 290645..301409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ642_RS01320 (RJ642_01320) yhhI 290727..291863 (-) 1137 WP_000420980.1 ISAs1-like element ISEc1 family transposase -
  RJ642_RS01325 (RJ642_01325) - 292004..292354 (-) 351 Protein_262 lyase -
  RJ642_RS01330 (RJ642_01330) yhhH 292459..292842 (-) 384 WP_001271686.1 protein YhhH -
  RJ642_RS01335 (RJ642_01335) rhsB 292814..294187 (-) 1374 Protein_264 rhs element protein RhsB -
  RJ642_RS01340 (RJ642_01340) nikR 294435..294836 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  RJ642_RS01345 (RJ642_01345) nikE 294842..295648 (-) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  RJ642_RS01350 (RJ642_01350) amiE 295645..296409 (-) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  RJ642_RS01355 (RJ642_01355) nikC 296409..297242 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  RJ642_RS01360 (RJ642_01360) nikB 297239..298183 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  RJ642_RS01365 (RJ642_01365) nikA 298183..299757 (-) 1575 WP_000953361.1 nickel ABC transporter substrate-binding protein -
  RJ642_RS01370 (RJ642_01370) acpT 299868..300455 (-) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=803065 RJ642_RS01350 WP_001136229.1 295645..296409(-) (amiE) [Escherichia coli strain TUM12357]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=803065 RJ642_RS01350 WP_001136229.1 295645..296409(-) (amiE) [Escherichia coli strain TUM12357]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398