Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   RJ645_RS01430 Genome accession   NZ_CP134366
Coordinates   322167..322931 (-) Length   254 a.a.
NCBI ID   WP_001136211.1    Uniprot ID   -
Organism   Escherichia coli strain TUM12368     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 317167..327931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ645_RS01405 (RJ645_01405) yhhJ 319010..320134 (+) 1125 WP_001314210.1 ABC transporter permease -
  RJ645_RS01410 (RJ645_01410) - 320207..320482 (+) 276 Protein_278 type II toxin-antitoxin system HicA family toxin -
  RJ645_RS01415 (RJ645_01415) - 320479..320838 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  RJ645_RS01420 (RJ645_01420) nikR 320958..321359 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  RJ645_RS01425 (RJ645_01425) nikE 321364..322170 (-) 807 WP_000173690.1 nickel import ATP-binding protein NikE -
  RJ645_RS01430 (RJ645_01430) amiE 322167..322931 (-) 765 WP_001136211.1 nickel import ATP-binding protein NikD Regulator
  RJ645_RS01435 (RJ645_01435) nikC 322931..323764 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  RJ645_RS01440 (RJ645_01440) nikB 323761..324705 (-) 945 WP_310984774.1 nickel ABC transporter permease subunit NikB -
  RJ645_RS01445 (RJ645_01445) nikA 324705..326279 (-) 1575 WP_022646227.1 nickel ABC transporter substrate-binding protein -
  RJ645_RS01450 (RJ645_01450) acpT 326390..326977 (-) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.45 Da        Isoelectric Point: 6.9016

>NTDB_id=802981 RJ645_RS01430 WP_001136211.1 322167..322931(-) (amiE) [Escherichia coli strain TUM12368]
MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=802981 RJ645_RS01430 WP_001136211.1 322167..322931(-) (amiE) [Escherichia coli strain TUM12368]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTTAA
ACGCGGGCGTGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCACTGACCTGCGCCGCGACGCTGGGCATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGTGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTTCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCACGCGTGC
TGAAGCTGTACCCGTTTGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGTTGTGTGAATCGCCG
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATTCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACACGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAATGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398