Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   RJ649_RS01215 Genome accession   NZ_CP134362
Coordinates   265486..266250 (-) Length   254 a.a.
NCBI ID   WP_001136232.1    Uniprot ID   Q0TBX9
Organism   Escherichia coli strain TUM13754     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 260486..271250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ649_RS01200 (RJ649_01200) yhhJ 263143..264267 (+) 1125 WP_001297994.1 ABC transporter permease -
  RJ649_RS01205 (RJ649_01205) nikR 264276..264677 (-) 402 WP_001190060.1 nickel-responsive transcriptional regulator NikR -
  RJ649_RS01210 (RJ649_01210) nikE 264683..265489 (-) 807 WP_000173679.1 nickel import ATP-binding protein NikE -
  RJ649_RS01215 (RJ649_01215) amiE 265486..266250 (-) 765 WP_001136232.1 nickel import ATP-binding protein NikD Regulator
  RJ649_RS01220 (RJ649_01220) nikC 266250..267083 (-) 834 WP_001008959.1 nickel ABC transporter permease subunit NikC -
  RJ649_RS01225 (RJ649_01225) nikB 267080..268024 (-) 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -
  RJ649_RS01230 (RJ649_01230) nikA 268024..269598 (-) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  RJ649_RS01235 (RJ649_01235) acpT 269709..270296 (-) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=802893 RJ649_RS01215 WP_001136232.1 265486..266250(-) (amiE) [Escherichia coli strain TUM13754]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=802893 RJ649_RS01215 WP_001136232.1 265486..266250(-) (amiE) [Escherichia coli strain TUM13754]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGAAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CTGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0TBX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398