Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   L6478_RS05935 Genome accession   NZ_AP022970
Coordinates   1146652..1150167 (+) Length   1171 a.a.
NCBI ID   WP_236787788.1    Uniprot ID   -
Organism   Bacillus cereus strain J75     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1141652..1155167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6478_RS05910 (BCJMU75_1079) - 1141931..1143160 (+) 1230 WP_236787786.1 ATP-binding protein -
  L6478_RS05915 (BCJMU75_1080) - 1143205..1143864 (+) 660 WP_042874587.1 response regulator transcription factor -
  L6478_RS05920 (BCJMU75_1081) - 1144139..1144720 (+) 582 WP_062922391.1 hypothetical protein -
  L6478_RS05925 (BCJMU75_1083) - 1145322..1145915 (+) 594 WP_000347516.1 TVP38/TMEM64 family protein -
  L6478_RS05930 (BCJMU75_1084) lepB 1145972..1146535 (+) 564 WP_000751905.1 signal peptidase I -
  L6478_RS05935 (BCJMU75_1085) addB 1146652..1150167 (+) 3516 WP_236787788.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  L6478_RS05940 (BCJMU75_1086) addA 1150164..1153889 (+) 3726 WP_062922388.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  L6478_RS05945 (BCJMU75_1087) - 1153904..1154191 (+) 288 WP_062922387.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  L6478_RS05950 (BCJMU75_1088) - 1154282..1154659 (-) 378 WP_236787790.1 DUF1433 domain-containing protein -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134392.61 Da        Isoelectric Point: 5.3733

>NTDB_id=80285 L6478_RS05935 WP_236787788.1 1146652..1150167(+) (addB) [Bacillus cereus strain J75]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEIWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVKGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEMEKTIPLMEQPRFHSPALAHLEMHYEARPNEKFHGEASVKIHTAANL
RAEVEGVAREIRRLVAEENYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFIMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSTVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKSLDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGHAASPAGVLYFHIHNPIVEVKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=80285 L6478_RS05935 WP_236787788.1 1146652..1150167(+) (addB) [Bacillus cereus strain J75]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAATTAAA
ACAGCGCCCAAGAGGGGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGGCA
GTGAGGATGTTAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTATTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTTCATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAACAAAACGGCTTCTTTGAACATCTCGGCAGTATGATTGCGGAGTTTAAACGATATA
ACGTGACGCCATCAAACGTATATGAAATTTGGCAACAATTAGATGCGCATAGTAGCAGTGCAGAACAAAAGTTACTAGCG
AATAAAGTGTATGATTTACAACTACTATATGATGATTTTGAGCGTGCTTTAATCGGAAAGTATTTAGATTCAGAAGACTA
CCTGCAATTGCTAGTCGAAAAGCTTCCGCAATCTGAATATGTAAAGGGAGCTGAAATTTATATAGATGGATTTCACTCAT
TTTCACCGCAAGAGTTAGAAATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTTACAATCACGTTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAATTAGATTTATTTTATGAAACGACATTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATGGAAAAAACGATTCCACTTATGGAACAGCCGCGTTTTCATTCTCCGGCATTAGCGCATT
TAGAAATGCATTACGAAGCGCGTCCAAATGAAAAGTTTCACGGTGAAGCAAGTGTAAAGATTCATACAGCAGCTAATTTA
CGAGCGGAAGTAGAAGGCGTTGCTCGTGAAATTCGTAGACTTGTGGCTGAAGAAAACTATCGTTACCGAGATATTGCGGT
TCTTCTTCGTAACGGGGAAAGTTATTACGATGTAATGCGGACACTATTTACAGATTATAATATCCCGCACTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGTTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTCTTTCGCTGCGTGAAAACAGAGCTTTTATATCCACTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTATTGCTTAGCATACGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGTCGCT
ATCGTTCTCTTGACGATACGAACGGGATGATCACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGTGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGCGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
CTTATTTTTAGAAGAGCTGGACGTTCCAAAAAAATTAGAAGCATTACGTATTCGTGCAGAAGAGAGTGGGGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTTATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTCGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTGATTGCTAATATTGATCGTTCAAGATTATCAAATGTGAAAGCAACATTTGTTATTGGCGTGAATGAAGGTG
TCATTCCAGCAGCACCGATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGATGTTTTGAGCGCTGCAGGTATTGAACTC
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCATTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
TATTTCATGCCCGCTTGCAGATGAGGAAGGGAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAATGACGTAAATGATTTATCACGTTCGGAACAAATTTCATACGTAGCAACGCCG
GAAGTAACACTGTCATATGTTATGCAGCAACTTCAAACGTGGAAGCGATATGGATTTGAAGGGAATTTAGACTTTTGGTG
GGATGTATATAATTTCTACGTAACTTCGGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGTTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGCTGTAAGTAGAGATTTATATGGAGACAAAATAAAAGGAAGCGTTTCTCGTATGGAA
TTATTTAACCGTTGTGCGTACGCTCATTTCGCGCAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTTAAACTTGATGC
GCCAGATATCGGGGAGCTTTTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAATCGTACTTGGGCAG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTACTGTAGTAATAGAAGAGATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAACA
TGCGAAGTCTAGCGGTTTCGTACCAGTTGATTTAGAAGTGCCATTCGGTATGGGCGGTACAGGATCACTTCCGCCAATGG
AATTCTCGTTACCTAATGGTGTAAAGATGGAAGTAGTTGGTCGTATTGACCGCGTTGATAAGGCGGAAGATGAAAACGGA
ACATTCCTCCGTATTATTGACTATAAATCAAGCTCAAAATCGTTAGACTTAACGGAAGTGTATTACGGATTGGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAAGGTCACGCAGCATCACCAGCTGGTG
TATTATATTTCCACATTCATAACCCAATTGTTGAGGTAAAAGGTGACGCATCTGAAGCAGAAATTGAAAAAGAAATTTTA
AAGAAATTCAAAATGAAAGGTCTCGTACTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGTTTTAGTGCGCGTTCTAGTATTGCCAGTGAACAAGAGTTTA
ACGTCCTGCAAAAATACGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAGGGTGTTATCGATATTGCTCCT
TACAAAAAGGGGAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCAGTTTGTCAGTTCGATGAATCACTTGAAGATAA
CCAGTTCCGTACGTTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(304-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

48.976

100

0.49

Multiple sequence alignment