Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RHT76_RS02245 Genome accession   NZ_CP133919
Coordinates   488472..488939 (-) Length   155 a.a.
NCBI ID   WP_000340159.1    Uniprot ID   -
Organism   Escherichia coli strain M21170     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 483472..493939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHT76_RS02220 (RHT76_02220) rpsG 483613..484083 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  RHT76_RS02225 (RHT76_02225) fusA 484180..486294 (+) 2115 WP_000124703.1 elongation factor G -
  RHT76_RS02230 (RHT76_02230) tuf 486365..487549 (+) 1185 WP_000031783.1 elongation factor Tu -
  RHT76_RS02235 (RHT76_02235) bfd 487732..487926 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  RHT76_RS02240 (RHT76_02240) bfr 487999..488475 (+) 477 WP_000675515.1 bacterioferritin -
  RHT76_RS02245 (RHT76_02245) pilD 488472..488939 (-) 468 WP_000340159.1 prepilin peptidase Machinery gene
  RHT76_RS02250 (RHT76_02250) rpsJ 489318..489629 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  RHT76_RS02255 (RHT76_02255) rplC 489662..490291 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  RHT76_RS02260 (RHT76_02260) rplD 490302..490907 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  RHT76_RS02265 (RHT76_02265) rplW 490904..491206 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  RHT76_RS02270 (RHT76_02270) rplB 491224..492045 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  RHT76_RS02275 (RHT76_02275) rpsS 492062..492340 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  RHT76_RS02280 (RHT76_02280) rplV 492355..492687 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  RHT76_RS02285 (RHT76_02285) rpsC 492705..493406 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  RHT76_RS02290 (RHT76_02290) rplP 493419..493829 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17034.17 Da        Isoelectric Point: 8.1255

>NTDB_id=801623 RHT76_RS02245 WP_000340159.1 488472..488939(-) (pilD) [Escherichia coli strain M21170]
MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=801623 RHT76_RS02245 WP_000340159.1 488472..488939(-) (pilD) [Escherichia coli strain M21170]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACATGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGCTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.882

81.935

0.368