Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   P0S91_RS23330 Genome accession   NZ_CP119968
Coordinates   4860031..4861158 (-) Length   375 a.a.
NCBI ID   WP_105220073.1    Uniprot ID   A0A6N8FRP1
Organism   Gloeocapsopsis dulcis strain AAB1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4855031..4866158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P0S91_RS23310 (P0S91_23250) - 4855206..4855490 (-) 285 WP_099702493.1 HU family DNA-binding protein -
  P0S91_RS23315 (P0S91_23255) - 4855823..4857934 (-) 2112 WP_105220076.1 type IV pilus secretin family protein -
  P0S91_RS23320 (P0S91_23260) - 4858361..4859116 (-) 756 WP_105220075.1 pilus assembly protein PilO -
  P0S91_RS23325 (P0S91_23265) - 4859113..4859964 (-) 852 WP_105220074.1 PilN domain-containing protein -
  P0S91_RS23330 (P0S91_23270) pilM 4860031..4861158 (-) 1128 WP_105220073.1 type IV pilus assembly protein PilM Machinery gene
  P0S91_RS23335 (P0S91_23275) - 4861277..4862602 (+) 1326 WP_105220072.1 ABC transporter substrate-binding protein -
  P0S91_RS23340 (P0S91_23280) - 4862849..4863382 (-) 534 WP_105220071.1 single-stranded DNA-binding protein -
  P0S91_RS23345 (P0S91_23285) - 4863498..4866056 (-) 2559 WP_105220070.1 mannose-1-phosphate guanyltransferase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41560.00 Da        Isoelectric Point: 4.5799

>NTDB_id=801285 P0S91_RS23330 WP_105220073.1 4860031..4861158(-) (pilM) [Gloeocapsopsis dulcis strain AAB1]
MFNRFQLLPNRFQLLPKRSKGVGIELAPERINIAQLRKQGQGIRLVAFSSVPVPEGIFQDGQIIDSPALAEIIQSALAQS
KIKAKRVATAVPGRDSLVRLIPLPAELDDQELREMVLNHEAGLYLPYPREEADVDYQKLGYFVDEDGIEKVQILLVATRK
EVTDIYIDTFQRAGLQVDVLEINSFALLRTIREKLREFPSQEAVVLVDIEFDNTEIAIIVDGIPQFSRTVPIGTFHLQNA
LLRALHLPVSRDTQVMLQSVVIPSTTTDEMGGAINTIDPGTAAMLRVMGELIDELRRSLDFYLSQSENLEVAQILLAGPG
GGLVNLDEFFQQRVNIPTLQIDPVAALSLEVDEKKISPGQRPGLGIVLGLGMREI

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=801285 P0S91_RS23330 WP_105220073.1 4860031..4861158(-) (pilM) [Gloeocapsopsis dulcis strain AAB1]
GTGTTTAACCGCTTCCAATTACTACCTAACCGCTTCCAACTACTACCAAAACGTAGCAAGGGAGTTGGCATTGAACTTGC
TCCCGAACGCATTAATATAGCTCAGCTACGTAAACAAGGTCAGGGAATAAGACTGGTAGCGTTTTCTTCAGTTCCCGTAC
CCGAAGGAATTTTTCAAGATGGTCAGATTATTGATTCACCAGCGCTAGCAGAAATTATCCAATCAGCCTTAGCCCAGAGC
AAAATTAAAGCGAAGCGGGTTGCTACTGCTGTGCCTGGAAGAGACTCGTTAGTGCGTTTGATTCCCTTACCTGCTGAGTT
AGATGACCAAGAATTACGTGAGATGGTCTTAAATCATGAAGCAGGTTTGTATTTGCCTTATCCTCGTGAAGAAGCGGATG
TAGATTATCAGAAACTAGGATACTTTGTGGATGAGGATGGCATTGAGAAGGTACAAATTTTACTCGTTGCTACCCGCAAA
GAAGTAACAGATATTTACATTGATACATTTCAACGAGCTGGGCTACAAGTTGATGTTTTAGAAATTAATAGTTTTGCCTT
GTTACGTACAATTCGCGAGAAATTACGTGAATTTCCTTCGCAGGAAGCCGTTGTTTTGGTTGATATCGAATTTGACAACA
CAGAAATTGCAATTATTGTAGATGGAATACCACAATTTTCGCGGACTGTCCCGATTGGTACATTTCATTTGCAAAATGCG
CTGTTGCGGGCACTGCATTTACCAGTGTCTAGAGACACGCAAGTTATGCTACAGTCAGTGGTCATTCCCAGCACGACAAC
AGATGAAATGGGAGGAGCGATCAATACTATCGATCCTGGTACTGCAGCAATGCTGCGAGTGATGGGAGAGTTAATTGATG
AATTACGGCGATCGCTCGATTTTTATCTCAGTCAAAGTGAAAATTTAGAAGTTGCTCAAATACTCTTGGCAGGACCTGGT
GGAGGATTAGTAAACTTAGATGAGTTTTTCCAACAACGAGTAAATATTCCCACCCTACAAATCGATCCTGTGGCAGCTTT
GTCTTTAGAAGTTGACGAGAAAAAAATTTCGCCTGGACAACGACCAGGATTAGGAATCGTTCTCGGTTTAGGAATGCGCG
AGATTTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(290-374)

Type IV pilus assembly protein PilM; (PilM_2)

(23-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N8FRP1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

63.369

99.733

0.632