Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   RDJ20_RS29680 Genome accession   NZ_CP133755
Coordinates   6192287..6192883 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain BJ86     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6187287..6197883
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RDJ20_RS29660 (RDJ20_29660) aroK 6188418..6188936 (-) 519 WP_003095833.1 shikimate kinase AroK -
  RDJ20_RS29665 (RDJ20_29665) pilQ 6188948..6191092 (-) 2145 WP_042170908.1 type 4a pilus secretin PilQ Machinery gene
  RDJ20_RS29670 (RDJ20_29670) comL/pilP 6191146..6191670 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  RDJ20_RS29675 (RDJ20_29675) pilO 6191667..6192290 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  RDJ20_RS29680 (RDJ20_29680) pilN 6192287..6192883 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  RDJ20_RS29685 (RDJ20_29685) pilM 6192883..6193947 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  RDJ20_RS29690 (RDJ20_29690) - 6194132..6196603 (+) 2472 WP_033977033.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=800941 RDJ20_RS29680 WP_003095839.1 6192287..6192883(-) (pilN) [Pseudomonas aeruginosa strain BJ86]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=800941 RDJ20_RS29680 WP_003095839.1 6192287..6192883(-) (pilN) [Pseudomonas aeruginosa strain BJ86]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCGGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAGTTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGGCGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGTTGACCGCTCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGTGCAGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369