Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   RG643_RS27690 Genome accession   NZ_CP133753
Coordinates   5919403..5919828 (-) Length   141 a.a.
NCBI ID   WP_033937140.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain ZYPA187     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5914403..5924828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG643_RS27670 (RG643_27665) ileS 5914560..5917391 (+) 2832 WP_003094730.1 isoleucine--tRNA ligase -
  RG643_RS27675 (RG643_27670) lspA 5917384..5917893 (+) 510 WP_003110420.1 signal peptidase II -
  RG643_RS27680 (RG643_27675) fkpB 5917886..5918326 (+) 441 WP_023108763.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  RG643_RS27685 (RG643_27680) ispH 5918412..5919356 (+) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  RG643_RS27690 (RG643_27685) comF 5919403..5919828 (-) 426 WP_033937140.1 type 4a pilus minor pilin PilE Machinery gene
  RG643_RS27695 (RG643_27690) pilY2 5919825..5920172 (-) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  RG643_RS27700 (RG643_27695) pilY1 5920174..5923665 (-) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  RG643_RS27705 (RG643_27700) pilX 5923677..5924264 (-) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=800838 RG643_RS27690 WP_033937140.1 5919403..5919828(-) (comF) [Pseudomonas aeruginosa strain ZYPA187]
MRTRQKGFTLLEMVVVVAVISILLGIAIPGYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=800838 RG643_RS27690 WP_033937140.1 5919403..5919828(-) (comF) [Pseudomonas aeruginosa strain ZYPA187]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCAGCATCCTCCTCGGCATCGC
CATTCCCGGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGGCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

40.625

90.78

0.369