Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RG643_RS21630 Genome accession   NZ_CP133753
Coordinates   4617970..4619754 (+) Length   594 a.a.
NCBI ID   WP_034039688.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain ZYPA187     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4612970..4624754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG643_RS21610 (RG643_21605) argE 4613210..4614364 (-) 1155 WP_003100077.1 acetylornithine deacetylase -
  RG643_RS21615 (RG643_21610) - 4614464..4615732 (-) 1269 WP_003100079.1 inorganic phosphate transporter -
  RG643_RS21620 (RG643_21615) - 4615777..4616454 (-) 678 WP_003100081.1 TIGR00153 family protein -
  RG643_RS21625 (RG643_21620) - 4616568..4617932 (+) 1365 WP_003100082.1 inorganic triphosphatase -
  RG643_RS21630 (RG643_21625) pilB 4617970..4619754 (+) 1785 WP_034039688.1 GspE/PulE family protein Machinery gene
  RG643_RS21635 (RG643_21630) - 4619976..4620428 (+) 453 WP_003107402.1 NfeD family protein -
  RG643_RS21640 (RG643_21635) - 4620560..4620889 (+) 330 WP_003118034.1 DUF2388 domain-containing protein -
  RG643_RS21645 (RG643_21640) gcvP 4620924..4623800 (-) 2877 WP_019727023.1 aminomethyl-transferring glycine dehydrogenase -
  RG643_RS21650 (RG643_21645) gcvH 4623974..4624363 (-) 390 WP_003107397.1 glycine cleavage system protein GcvH -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 66009.62 Da        Isoelectric Point: 6.5096

>NTDB_id=800810 RG643_RS21630 WP_034039688.1 4617970..4619754(+) (pilB) [Pseudomonas aeruginosa strain ZYPA187]
MSVFATAAQDRLLDLGDLLRELVAQGRVGQEQAEQCLAIRRSAVRNQQHPLEFLAAQQVEDLKRPGRKLDLETLTQWLAE
YAGQPYLRIDPLKIDVAAVTPLMSYAFAQRHGILAVALSPEEVTIASAQPFVQGWESNLVHVLKRPIKRVVANPADIQRY
TVEFFRLARSVSGATSPDQKISGVGNFEQLLNLGAADQEPDANDAHIVTIVDWLFQYAFQQRASDIHIEPRREQGTVRFR
IDGVLHNVYQFPPQVTMAVVSRLKSLGRMNVAEKRKPQDGRVKTKTPDGAEVELRLSTLPTAFGEKMVMRIFDPEVLLKS
FDQLGFSADDLRRWQSMTSQPNGIILVTGPTGSGKTTTLYTTLKQLATDEVNVCTIEDPIEMIEPSFNQMQVQHNIDLTF
ASGVRALLRQDPDIIMVGEIRDLETAEMAIQAALTGHLVLSTLHTNDSPTAITRLLELGVPYYLIRATVLGVMAQRLVRT
LCPHCRAPMQLADSDWHELTRPWSAPPPSGAHQAVGCLECRDTGYRGRAGVYEIMLLSDSLKELITADTDLVALRRQAYK
EGTRSLRLSGAQKVAAGISTLEEVLRVTPQSEQK

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=800810 RG643_RS21630 WP_034039688.1 4617970..4619754(+) (pilB) [Pseudomonas aeruginosa strain ZYPA187]
ATGTCCGTGTTCGCCACCGCCGCCCAGGACCGCCTGCTGGACCTCGGCGACCTGCTCCGCGAGCTGGTCGCCCAGGGCCG
GGTCGGGCAGGAGCAGGCCGAACAGTGCCTGGCGATCCGCCGCAGCGCGGTGCGCAACCAGCAGCATCCGCTGGAGTTCC
TCGCCGCGCAGCAGGTCGAGGATCTCAAGCGCCCCGGACGCAAGCTCGATCTGGAGACCCTCACCCAGTGGCTGGCCGAG
TACGCCGGCCAGCCCTACCTGCGCATCGACCCGCTGAAGATCGACGTCGCCGCGGTGACTCCGCTGATGTCCTACGCCTT
CGCCCAGCGCCATGGCATCCTCGCCGTCGCGCTGAGTCCGGAGGAAGTCACCATCGCCAGCGCCCAGCCCTTCGTACAGG
GCTGGGAAAGCAACCTGGTGCATGTGCTCAAGCGACCGATCAAGCGGGTGGTGGCGAACCCGGCGGACATCCAGCGCTAC
ACCGTCGAGTTCTTCCGCCTGGCCCGCTCGGTCAGCGGCGCCACCTCGCCGGACCAGAAGATCAGCGGCGTCGGCAACTT
CGAACAGTTGCTCAACCTCGGCGCCGCCGACCAGGAGCCGGACGCCAACGACGCGCACATCGTCACCATCGTCGACTGGC
TGTTCCAGTACGCCTTCCAGCAGCGCGCCAGCGACATCCACATCGAGCCGCGCCGCGAGCAGGGCACCGTGCGCTTCCGT
ATCGACGGCGTGCTGCACAACGTCTACCAGTTCCCGCCGCAGGTGACCATGGCGGTGGTCAGCCGCCTGAAGTCGCTGGG
ACGGATGAACGTGGCGGAGAAACGCAAGCCGCAGGACGGCCGGGTCAAGACCAAGACCCCGGACGGCGCCGAGGTGGAGC
TGCGCCTGTCGACCCTGCCCACCGCCTTCGGCGAGAAGATGGTGATGCGCATCTTCGACCCCGAGGTGCTGCTCAAGAGC
TTCGACCAGCTCGGCTTCTCCGCCGACGACCTGCGCCGCTGGCAGAGCATGACCAGCCAGCCCAACGGCATCATCCTGGT
CACCGGCCCCACCGGCTCGGGCAAGACCACCACGCTCTACACCACGCTCAAGCAACTGGCCACCGATGAGGTGAACGTCT
GCACCATCGAGGACCCGATCGAGATGATCGAGCCCTCGTTCAACCAGATGCAGGTCCAGCACAACATCGACCTGACCTTC
GCCAGCGGCGTACGCGCCCTGCTGCGGCAGGACCCGGACATCATCATGGTCGGCGAGATCCGCGACCTGGAGACCGCCGA
GATGGCGATCCAGGCGGCGCTCACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACGACTCCCCCACGGCGATCACCC
GCCTGCTCGAACTGGGCGTACCCTACTACCTGATCCGCGCCACGGTGCTAGGGGTCATGGCCCAACGCCTGGTGCGCACG
CTCTGCCCGCACTGCAGGGCGCCGATGCAACTGGCGGACAGCGACTGGCACGAACTGACCCGCCCCTGGAGCGCCCCGCC
GCCCAGCGGCGCGCACCAGGCCGTGGGTTGCCTGGAGTGCCGCGACACCGGCTATCGCGGGCGCGCCGGCGTCTATGAAA
TCATGCTGCTCAGCGATAGCCTGAAAGAACTGATCACCGCCGACACCGACCTGGTCGCCCTGCGCCGCCAGGCGTACAAG
GAAGGCACCCGCAGCCTGCGCCTGTCCGGCGCGCAGAAGGTCGCCGCAGGTATCAGTACGCTGGAGGAAGTACTGCGGGT
GACCCCGCAGAGCGAACAGAAGTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(216-480)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(69-167)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.495

90.067

0.374