Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   RG463_RS30455 Genome accession   NZ_CP133738
Coordinates   6411636..6412232 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain FI-17645     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6406636..6417232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG463_RS30435 (RG463_30435) aroK 6407767..6408285 (-) 519 WP_003095833.1 shikimate kinase AroK -
  RG463_RS30440 (RG463_30440) pilQ 6408297..6410441 (-) 2145 WP_003095835.1 type 4a pilus secretin PilQ Machinery gene
  RG463_RS30445 (RG463_30445) comL/pilP 6410495..6411019 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  RG463_RS30450 (RG463_30450) pilO 6411016..6411639 (-) 624 WP_003095838.1 type 4a pilus biogenesis protein PilO Machinery gene
  RG463_RS30455 (RG463_30455) pilN 6411636..6412232 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  RG463_RS30460 (RG463_30460) pilM 6412232..6413296 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  RG463_RS30465 (RG463_30465) - 6413481..6415952 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=800635 RG463_RS30455 WP_003095839.1 6411636..6412232(-) (pilN) [Pseudomonas aeruginosa strain FI-17645]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=800635 RG463_RS30455 WP_003095839.1 6411636..6412232(-) (pilN) [Pseudomonas aeruginosa strain FI-17645]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCGGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGATTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGTCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATTGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGTTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369