Detailed information    

insolico Bioinformatically predicted

Overview

Name   mutS/mutS2   Type   Machinery gene
Locus tag   RFN65_RS06665 Genome accession   NZ_CP133703
Coordinates   1315975..1318332 (+) Length   785 a.a.
NCBI ID   WP_063335830.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP35     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1310975..1323332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS06645 (RFN65_06645) rnhC 1312296..1313237 (-) 942 WP_100506472.1 ribonuclease HIII -
  RFN65_RS06650 (RFN65_06650) zapA 1313371..1313628 (+) 258 WP_003229534.1 cell division protein ZapA -
  RFN65_RS06655 (RFN65_06655) yshB 1313635..1314168 (+) 534 WP_003229537.1 CvpA family protein -
  RFN65_RS06660 (RFN65_06660) polX 1314242..1315954 (+) 1713 WP_063335829.1 DNA polymerase/3'-5' exonuclease PolX -
  RFN65_RS06665 (RFN65_06665) mutS/mutS2 1315975..1318332 (+) 2358 WP_063335830.1 endonuclease MutS2 Machinery gene
  RFN65_RS06670 (RFN65_06670) yshE 1318347..1318751 (+) 405 WP_003237674.1 DUF350 domain-containing protein -
  RFN65_RS06675 (RFN65_06675) lcfA 1318940..1320622 (+) 1683 WP_309530178.1 long-chain-fatty-acid--CoA ligase LcfA -
  RFN65_RS06680 (RFN65_06680) fadR 1320727..1321311 (+) 585 WP_100506475.1 fatty acid metabolism transcriptional regulator FadR -
  RFN65_RS06685 (RFN65_06685) fadB 1321326..1322102 (+) 777 WP_003229549.1 enoyl-CoA hydratase -
  RFN65_RS06690 (RFN65_06690) etfB 1322117..1322890 (+) 774 WP_014480500.1 electron transfer flavoprotein subunit beta -

Sequence

Protein


Download         Length: 785 a.a.        Molecular weight: 87296.63 Da        Isoelectric Point: 6.1852

>NTDB_id=800103 RFN65_RS06665 WP_063335830.1 1315975..1318332(+) (mutS/mutS2) [Bacillus subtilis strain CP35]
MQQKVLSALEFHKVKEQVIGHAASSLGKEMLLELKPSASIDEIKKQLDEVDEASDIIRLRGQAPFGGLVDIRGALRRAEI
GSVLSPSEFTEISGLLYAVKQMKHFITQMAEDGVDIPLIHQHAEQLITLSDLERDINSCIDDHGEVLDHASETLRGIRTQ
LRTLESRVRDRLESMLRSSSASKMLSDTIVTIRNDRFVIPVKQEYRSSYGGIVHDTSSSGATLFIEPQAIVDMNNSLQQA
KVKEKQEIERILRVLTEKTAEHTEELFLDLQVLQTLDFIFAKARYAKAVKATKPIMNDTGFIRLKKARHPLLPPDQVVAN
DIELGRDFSTIVITGPNTGGKTVTLKTLGLLTLMAQSGLHIPADEGSEAAVFEHVFADIGDEQSIEQSLSTFSSHMVNIV
GILEQVNENSLVLFDELGAGTDPQEGAALAMSILDDVHRTNARVLATTHYPELKAYGYNREGVMNASVEFDIETLSPTYK
LLIGVPGRSNAFEISKRLGLPDHIIGQAKSEMTAEHNEVDTMIASLEQSKKRAEEELSETESIRKEAEKLHKELQQQIIE
LNSKKDKMLEDAEQQAAEKVKAAIKEAEDIIHELRSIKEEHKSFKDHELINAKKRLEGAMPAFEKSKKPEKPKAQKRDFK
PGDEVKVLTFGQKGTLLEKTGGNEWNVQIGILKMKVKEKDLEFIKSAPEPKKEKIITAVKGKDYHVSLELDLRGERYENA
LSRVEKYLDDAVLAGYPRVSIIHGKGTGALRKGVQDLLKNHRSVKSSRFGEAGEGGSGVTVVELK

Nucleotide


Download         Length: 2358 bp        

>NTDB_id=800103 RFN65_RS06665 WP_063335830.1 1315975..1318332(+) (mutS/mutS2) [Bacillus subtilis strain CP35]
GTGCAGCAAAAAGTATTATCAGCTCTTGAATTTCATAAAGTGAAAGAACAGGTTATTGGGCATGCCGCTTCATCGCTCGG
AAAAGAAATGCTTCTCGAGCTTAAGCCTTCTGCTTCTATAGACGAAATCAAAAAACAGCTTGATGAAGTAGACGAAGCTT
CTGACATTATCCGGCTGAGAGGCCAAGCGCCATTTGGCGGCCTTGTAGATATCAGAGGAGCGTTAAGACGGGCGGAAATC
GGCAGCGTTCTCAGTCCTTCTGAATTCACTGAAATCTCAGGCCTGCTTTATGCAGTTAAACAAATGAAACATTTTATCAC
CCAAATGGCTGAAGACGGTGTCGACATTCCGCTGATCCATCAGCATGCTGAACAGCTTATCACGCTGTCCGATTTAGAGC
GGGACATTAATTCCTGCATTGATGATCACGGAGAAGTGCTTGATCATGCATCGGAAACATTAAGAGGAATCCGCACACAG
CTCAGAACACTCGAATCAAGAGTCAGAGACCGGTTAGAGTCGATGCTGCGTTCCTCTTCCGCTTCGAAAATGCTGTCTGA
TACGATTGTTACGATTCGGAATGACCGCTTTGTGATCCCGGTCAAACAGGAGTACAGATCCAGCTATGGAGGAATTGTGC
ACGACACCTCATCCTCTGGTGCGACACTATTCATTGAACCGCAGGCGATTGTAGATATGAACAATTCCCTTCAGCAGGCG
AAAGTGAAAGAAAAGCAAGAAATTGAACGGATTTTGCGTGTGCTGACAGAGAAAACGGCAGAGCATACAGAGGAGCTATT
TCTAGATTTGCAAGTGCTGCAGACGCTTGACTTTATTTTTGCAAAAGCTAGATATGCAAAAGCGGTTAAAGCGACAAAAC
CGATTATGAACGACACCGGCTTTATCCGTTTGAAAAAAGCCCGCCATCCATTGCTTCCGCCTGACCAGGTTGTTGCCAAT
GACATCGAGCTTGGCCGCGATTTTTCAACAATTGTCATCACAGGGCCAAACACCGGGGGGAAAACAGTCACCCTTAAAAC
GTTAGGCCTGCTAACCTTAATGGCGCAATCAGGTCTTCATATCCCGGCAGATGAAGGGTCAGAAGCGGCAGTATTTGAGC
ACGTATTCGCTGATATCGGTGATGAACAGTCGATTGAGCAAAGTTTAAGTACGTTCTCATCCCATATGGTGAACATTGTC
GGCATTTTAGAACAGGTCAATGAAAACAGCCTTGTGCTTTTCGATGAACTTGGTGCAGGGACAGATCCGCAGGAGGGGGC
GGCCCTCGCCATGAGCATCTTGGATGACGTGCATCGCACCAATGCACGAGTGTTAGCTACGACGCATTATCCGGAATTGA
AGGCGTACGGCTATAACAGAGAAGGCGTCATGAATGCCAGCGTTGAATTTGACATTGAAACGCTGTCACCGACCTATAAA
CTTTTAATTGGTGTGCCGGGTCGAAGCAATGCTTTCGAAATTTCAAAACGCCTCGGGCTTCCGGACCATATCATCGGGCA
GGCGAAGTCAGAAATGACGGCCGAGCATAACGAAGTCGATACGATGATTGCGTCGCTGGAACAAAGCAAAAAACGTGCGG
AAGAAGAGCTTTCTGAGACAGAATCAATCAGAAAAGAAGCGGAAAAACTGCATAAAGAGCTGCAGCAGCAAATCATCGAG
CTTAACAGCAAAAAAGACAAAATGCTTGAAGATGCAGAACAGCAGGCTGCTGAAAAAGTAAAAGCGGCAATAAAAGAAGC
CGAGGACATTATTCATGAATTGCGCTCCATAAAAGAAGAACACAAATCCTTCAAGGATCACGAGCTGATTAACGCGAAGA
AACGGTTAGAAGGCGCTATGCCGGCTTTTGAAAAGTCCAAGAAACCGGAAAAGCCAAAAGCGCAAAAACGCGACTTTAAG
CCTGGTGACGAGGTGAAAGTCCTCACTTTCGGGCAAAAAGGAACATTGCTCGAAAAAACAGGCGGCAATGAATGGAATGT
TCAAATCGGTATTTTAAAGATGAAAGTAAAAGAAAAAGATCTGGAGTTTATCAAATCAGCTCCGGAGCCAAAAAAAGAAA
AAATCATTACAGCGGTCAAGGGAAAGGACTATCACGTATCGCTTGAACTTGATCTCCGCGGCGAACGCTATGAAAATGCC
CTCAGCCGGGTTGAAAAATACTTGGATGATGCGGTGTTAGCCGGATATCCAAGAGTGTCAATCATCCACGGAAAAGGAAC
CGGCGCTTTAAGAAAAGGCGTACAGGATCTTCTGAAAAACCACCGCAGCGTCAAAAGTTCCCGTTTCGGTGAAGCAGGTG
AGGGAGGATCAGGCGTTACGGTTGTTGAACTAAAATAA

Domains

Predicted by InterProScan.

Smr domain (Smr)

(710-785)

MutS domain V (MutS_V)

(331-512)

MutS2 and Smr-associated SH3 domain (MSSS)

(641-681)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mutS/mutS2 Bacillus subtilis subsp. subtilis str. 168

99.236

100

0.992