Detailed information    

insolico Bioinformatically predicted

Overview

Name   mutS/mutS2   Type   Machinery gene
Locus tag   RFN60_RS14300 Genome accession   NZ_CP133653
Coordinates   2757732..2760089 (-) Length   785 a.a.
NCBI ID   WP_015251513.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP38     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2752732..2765089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN60_RS14275 (RFN60_14275) etfB 2753173..2753946 (-) 774 WP_014480500.1 electron transfer flavoprotein subunit beta -
  RFN60_RS14280 (RFN60_14280) fadB 2753961..2754737 (-) 777 WP_003229549.1 enoyl-CoA hydratase -
  RFN60_RS14285 (RFN60_14285) fadR 2754752..2755336 (-) 585 WP_003229547.1 fatty acid metabolism transcriptional regulator FadR -
  RFN60_RS14290 (RFN60_14290) lcfA 2755441..2757123 (-) 1683 WP_004399166.1 long-chain-fatty-acid--CoA ligase LcfA -
  RFN60_RS14295 (RFN60_14295) yshE 2757313..2757717 (-) 405 WP_003237674.1 DUF350 domain-containing protein -
  RFN60_RS14300 (RFN60_14300) mutS/mutS2 2757732..2760089 (-) 2358 WP_015251513.1 endonuclease MutS2 Machinery gene
  RFN60_RS14305 (RFN60_14305) polX 2760110..2761822 (-) 1713 WP_003229538.1 DNA polymerase/3'-5' exonuclease PolX -
  RFN60_RS14310 (RFN60_14310) yshB 2761896..2762429 (-) 534 WP_003229537.1 CvpA family protein -
  RFN60_RS14315 (RFN60_14315) zapA 2762436..2762693 (-) 258 WP_003229534.1 cell division protein ZapA -
  RFN60_RS14320 (RFN60_14320) rnhC 2762827..2763765 (+) 939 WP_015251512.1 ribonuclease HIII -

Sequence

Protein


Download         Length: 785 a.a.        Molecular weight: 87402.78 Da        Isoelectric Point: 6.1361

>NTDB_id=799859 RFN60_RS14300 WP_015251513.1 2757732..2760089(-) (mutS/mutS2) [Bacillus subtilis strain CP38]
MQQKVLSALEFHKVKEQVIGHAASSLGKEMLLELKPSASIDEIKKQLDEVDEASDIIRLRGQAPFGGLVDIRGALRRAEI
GSVLSPSEFTEISGLLYAVKQMKHFITQMAEDGVDIPLIHQHAEQLITLSDLERDINSCIDDHGEVLDHASETLRGIRTQ
LRTLESRVRDRLESMLRSSSASKMLSDTIVTIRNDRFVIPVKQEYRSSYGGIVHDTSSSGATLFIEPQAIVDMNNSLQQA
KVKEKQEIERILRVLTEKTAEYTEELFLDLQVLQTLDFIFAKARYAKAVKATKPIMNDTGFIRLKKARHPLLPPDQVVAN
DIELGRDFSTIVITGPNTGGKTVTLKTLGLLTLMAQSGLHIPADEGSEAAVFEHVFADIGDEQSIEQSLSTFSSHMVNIV
GILEQVNENSLVLFDELGAGTDPQEGAALAMSILDDVHRTNARVLATTHYPELKAYGYNREGVMNASVEFDIETLSPTYK
LLIGVPGRSNAFEISKRLGLPDHIIGQAKSEMTAEHNEVDTMIASLEQSKKRAEEELSETESIRKEAEKLHKELQQQIIE
LNSKKDKMLEEAEQQAAEKVKAAMKEAEDIIHELRSIKEEHKSFKDHELINAKKRLEGAMPAFEKSKKPEKPKTQKRDFK
PGDEVKVLTFGQKGTLLEKTGGNEWNVQIGILKMKVKEKDLEFIKSAPEPKKEKMITAVKGKDYHVSLELDLRGERYENA
LSRVEKYLDDAVLAGYPRVSIIHGKGTGALRKGVQDLLKNHRSVKSSRFGEAGEGGSGVTVVELK

Nucleotide


Download         Length: 2358 bp        

>NTDB_id=799859 RFN60_RS14300 WP_015251513.1 2757732..2760089(-) (mutS/mutS2) [Bacillus subtilis strain CP38]
GTGCAGCAAAAAGTATTATCAGCTCTTGAATTTCATAAAGTGAAAGAACAGGTTATTGGGCATGCCGCTTCATCGCTCGG
AAAAGAAATGCTTCTCGAGCTTAAGCCTTCTGCTTCTATAGACGAAATCAAAAAACAGCTGGATGAAGTAGACGAAGCTT
CTGACATTATCCGGCTGAGAGGCCAAGCGCCATTTGGCGGCCTTGTAGATATCAGAGGAGCGTTAAGACGGGCGGAAATC
GGCAGCGTTCTCAGTCCTTCTGAATTCACTGAAATCTCAGGCCTGCTTTATGCAGTTAAACAAATGAAACATTTTATCAC
GCAAATGGCTGAAGACGGTGTCGACATTCCGCTGATCCATCAGCATGCTGAACAGCTTATCACGCTGTCCGATTTAGAGC
GGGACATTAATTCCTGCATTGATGATCACGGAGAAGTGCTTGATCATGCATCGGAAACATTAAGAGGAATCCGCACACAG
CTCAGAACACTCGAATCAAGAGTCAGAGACCGGTTAGAGTCGATGCTGCGTTCCTCTTCCGCATCGAAAATGCTGTCTGA
TACGATTGTTACGATTCGGAATGACCGCTTTGTGATCCCGGTCAAACAGGAGTACAGATCCAGCTATGGAGGAATTGTGC
ACGACACCTCATCCTCTGGTGCGACACTATTCATTGAACCGCAGGCGATTGTAGATATGAACAATTCCCTTCAGCAGGCG
AAAGTGAAAGAAAAGCAAGAAATTGAACGGATTTTGCGTGTGCTGACAGAGAAAACGGCAGAGTATACAGAGGAGCTATT
TCTAGATTTGCAAGTGCTGCAGACGCTTGACTTTATTTTTGCAAAAGCTAGATATGCAAAAGCGGTTAAAGCGACAAAAC
CGATTATGAACGACACCGGCTTTATCCGTTTGAAAAAAGCCCGCCATCCATTGCTTCCGCCTGATCAGGTTGTTGCCAAT
GACATCGAGCTTGGCCGCGATTTTTCAACCATTGTCATCACAGGGCCAAACACCGGGGGGAAAACAGTCACCCTTAAAAC
GTTAGGCCTGCTAACCTTAATGGCGCAGTCAGGTCTTCATATCCCGGCAGATGAAGGGTCAGAAGCGGCAGTATTTGAGC
ACGTATTCGCTGATATCGGTGATGAACAGTCGATTGAGCAAAGTTTAAGTACGTTCTCATCCCATATGGTGAATATTGTC
GGCATTTTAGAACAGGTCAATGAAAACAGCCTTGTGCTTTTCGATGAACTTGGTGCAGGGACAGATCCGCAGGAGGGGGC
GGCCCTCGCCATGAGCATCTTGGATGACGTGCATCGCACCAATGCACGAGTGTTAGCTACGACGCATTATCCGGAATTGA
AGGCGTACGGCTATAACAGAGAAGGCGTCATGAATGCCAGCGTTGAATTTGACATCGAAACGCTGTCACCGACCTATAAA
CTTTTAATTGGTGTGCCGGGTCGAAGCAATGCTTTCGAAATTTCAAAACGCCTCGGGCTCCCGGACCATATCATCGGGCA
GGCGAAGTCAGAAATGACGGCCGAGCATAACGAAGTCGATACGATGATTGCGTCGCTGGAACAAAGCAAAAAACGTGCGG
AAGAAGAGCTTTCTGAGACAGAATCAATCAGAAAAGAAGCGGAAAAACTGCATAAAGAGCTGCAGCAGCAAATCATCGAG
CTTAACAGCAAAAAAGACAAAATGCTTGAAGAGGCAGAACAGCAGGCTGCTGAAAAAGTAAAAGCGGCAATGAAAGAAGC
CGAGGACATTATTCATGAATTGCGCTCCATAAAAGAAGAACACAAATCCTTCAAGGATCACGAGCTGATTAACGCGAAGA
AACGGTTAGAAGGCGCTATGCCTGCTTTTGAAAAGTCCAAGAAACCGGAAAAGCCGAAAACGCAAAAACGCGACTTTAAG
CCTGGCGACGAGGTGAAAGTCCTCACTTTCGGGCAAAAAGGAACATTGCTCGAAAAAACAGGCGGCAATGAATGGAACGT
TCAAATCGGTATTTTAAAGATGAAAGTAAAAGAAAAAGATTTGGAGTTTATCAAATCAGCTCCGGAGCCAAAAAAAGAAA
AAATGATTACAGCGGTCAAAGGAAAGGACTATCACGTATCGCTTGAACTTGATCTTCGCGGCGAACGCTATGAAAATGCC
CTCAGCCGGGTTGAAAAATACTTGGATGATGCGGTGTTAGCCGGATATCCAAGAGTGTCAATCATCCACGGAAAAGGAAC
CGGCGCTTTAAGAAAAGGCGTACAGGATCTTCTGAAAAACCACCGCAGCGTCAAAAGTTCCCGTTTCGGTGAAGCAGGTG
AGGGAGGATCAGGCGTTACGGTTGTTGAACTAAAATAA

Domains

Predicted by InterProScan.

MutS domain V (MutS_V)

(331-512)

Smr domain (Smr)

(710-785)

MutS2 and Smr-associated SH3 domain (MSSS)

(641-681)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mutS/mutS2 Bacillus subtilis subsp. subtilis str. 168

99.873

100

0.999