Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   RB265_RS15965 Genome accession   NZ_CP133128
Coordinates   3074437..3075201 (+) Length   254 a.a.
NCBI ID   WP_001136210.1    Uniprot ID   Q8X5U1
Organism   Escherichia coli O157:H7 strain PNUSAE146743     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 3069437..3080201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RB265_RS15945 (RB265_15945) acpT 3070391..3070978 (+) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -
  RB265_RS15950 (RB265_15950) nikA 3071089..3072663 (+) 1575 WP_000953364.1 nickel ABC transporter substrate-binding protein -
  RB265_RS15955 (RB265_15955) nikB 3072663..3073607 (+) 945 WP_000947083.1 nickel ABC transporter permease subunit NikB -
  RB265_RS15960 (RB265_15960) nikC 3073604..3074437 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  RB265_RS15965 (RB265_15965) amiE 3074437..3075201 (+) 765 WP_001136210.1 nickel import ATP-binding protein NikD Regulator
  RB265_RS15970 (RB265_15970) nikE 3075198..3076004 (+) 807 WP_000173697.1 nickel import ATP-binding protein NikE -
  RB265_RS15975 (RB265_15975) nikR 3076010..3076411 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  RB265_RS15980 (RB265_15980) - 3076610..3077356 (+) 747 WP_001301676.1 GntR family transcriptional regulator -
  RB265_RS15985 (RB265_15985) - 3077381..3077854 (+) 474 WP_001161553.1 PTS sugar transporter subunit IIA -
  RB265_RS15990 (RB265_15990) - 3077851..3078132 (+) 282 WP_000084025.1 PTS sugar transporter subunit IIB -
  RB265_RS15995 (RB265_15995) - 3078209..3079567 (+) 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26790.39 Da        Isoelectric Point: 6.5997

>NTDB_id=798543 RB265_RS15965 WP_001136210.1 3074437..3075201(+) (amiE) [Escherichia coli O157:H7 strain PNUSAE146743]
MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=798543 RB265_RS15965 WP_001136210.1 3074437..3075201(+) (amiE) [Escherichia coli O157:H7 strain PNUSAE146743]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTGTCGTTAACCCTGAA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGAATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAGACGTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCACGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTTTGCGAATCACCG
TTTATCATCGCCGATGAACCAACCACCGACCTCGACGTGGTAGCACAGGCACGCATTCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAGGCGCCGGGGATGCTGCTGGTCACCCATGATATGGGCGTGGTGGCGCGTCTGGCGGATGACGTAGCGGTAA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTCGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8X5U1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398