Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RB265_RS15180 Genome accession   NZ_CP133128
Coordinates   2911481..2911948 (+) Length   155 a.a.
NCBI ID   WP_000381746.1    Uniprot ID   Q8X892
Organism   Escherichia coli O157:H7 strain PNUSAE146743     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2906481..2916948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RB265_RS15135 (RB265_15135) rplP 2906591..2907001 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  RB265_RS15140 (RB265_15140) rpsC 2907014..2907715 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  RB265_RS15145 (RB265_15145) rplV 2907733..2908065 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  RB265_RS15150 (RB265_15150) rpsS 2908080..2908358 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  RB265_RS15155 (RB265_15155) rplB 2908375..2909196 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  RB265_RS15160 (RB265_15160) rplW 2909214..2909516 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  RB265_RS15165 (RB265_15165) rplD 2909513..2910118 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  RB265_RS15170 (RB265_15170) rplC 2910129..2910758 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  RB265_RS15175 (RB265_15175) rpsJ 2910791..2911102 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  RB265_RS15180 (RB265_15180) pilD 2911481..2911948 (+) 468 WP_000381746.1 A24 family peptidase Machinery gene
  RB265_RS15185 (RB265_15185) bfr 2911945..2912421 (-) 477 WP_000675516.1 bacterioferritin -
  RB265_RS15190 (RB265_15190) bfd 2912494..2912688 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  RB265_RS15195 (RB265_15195) tuf 2912871..2914055 (-) 1185 WP_000031783.1 elongation factor Tu -
  RB265_RS15200 (RB265_15200) fusA 2914126..2916240 (-) 2115 WP_000124700.1 elongation factor G -
  RB265_RS15205 (RB265_15205) rpsG 2916337..2916807 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17099.29 Da        Isoelectric Point: 8.1343

>NTDB_id=798538 RB265_RS15180 WP_000381746.1 2911481..2911948(+) (pilD) [Escherichia coli O157:H7 strain PNUSAE146743]
MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHSWAFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=798538 RB265_RS15180 WP_000381746.1 2911481..2911948(+) (pilD) [Escherichia coli O157:H7 strain PNUSAE146743]
ATGGACACTACCCTTCCCTTTCTAATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTCATCCTGATGGTTTAGCCG
ATGCGTTATGGGGGGCAATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGCCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGCGCCTGGCATTCATGGGCCTTCTTACCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAATCATTAAAAAACCCGCTGC
CTTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTATAGGCTGGGATAGTTTGCTGATAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8X892

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

43.478

89.032

0.387