Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   RBH01_RS25375 Genome accession   NZ_CP132997
Coordinates   5428690..5429115 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain ZYPA162     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5423690..5434115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBH01_RS25360 (RBH01_25365) pilX 5424260..5424847 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  RBH01_RS25365 (RBH01_25370) pilY1 5424859..5428344 (+) 3486 WP_033940136.1 type 4a pilus biogenesis protein PilY1 -
  RBH01_RS25370 (RBH01_25375) pilY2 5428346..5428693 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  RBH01_RS25375 (RBH01_25380) comF 5428690..5429115 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  RBH01_RS25380 (RBH01_25385) ispH 5429162..5430106 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  RBH01_RS25385 (RBH01_25390) fkpB 5430192..5430632 (-) 441 WP_023108763.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  RBH01_RS25390 (RBH01_25395) lspA 5430625..5431134 (-) 510 WP_003110420.1 signal peptidase II -
  RBH01_RS25395 (RBH01_25400) ileS 5431127..5433958 (-) 2832 WP_096253161.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=797965 RBH01_RS25375 WP_003094721.1 5428690..5429115(+) (comF) [Pseudomonas aeruginosa strain ZYPA162]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=797965 RBH01_RS25375 WP_003094721.1 5428690..5429115(+) (comF) [Pseudomonas aeruginosa strain ZYPA162]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383