Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   RBH03_RS23875 Genome accession   NZ_CP132995
Coordinates   5143460..5143885 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain ZYPA192     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5138460..5148885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBH03_RS23855 (RBH03_23860) ileS 5138617..5141448 (+) 2832 WP_096253161.1 isoleucine--tRNA ligase -
  RBH03_RS23860 (RBH03_23865) lspA 5141441..5141950 (+) 510 WP_003110420.1 signal peptidase II -
  RBH03_RS23865 (RBH03_23870) fkpB 5141943..5142383 (+) 441 WP_023108763.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  RBH03_RS23870 (RBH03_23875) ispH 5142469..5143413 (+) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  RBH03_RS23875 (RBH03_23880) comF 5143460..5143885 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  RBH03_RS23880 (RBH03_23885) pilY2 5143882..5144229 (-) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  RBH03_RS23885 (RBH03_23890) pilY1 5144231..5147716 (-) 3486 WP_033940136.1 type 4a pilus biogenesis protein PilY1 -
  RBH03_RS23890 (RBH03_23895) pilX 5147728..5148315 (-) 588 WP_003094700.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=797866 RBH03_RS23875 WP_003094721.1 5143460..5143885(-) (comF) [Pseudomonas aeruginosa strain ZYPA192]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=797866 RBH03_RS23875 WP_003094721.1 5143460..5143885(-) (comF) [Pseudomonas aeruginosa strain ZYPA192]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383