Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   RBH02_RS24050 Genome accession   NZ_CP132993
Coordinates   5183346..5183771 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain SRMPA3860     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5178346..5188771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBH02_RS24035 (RBH02_24040) pilX 5178910..5179497 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  RBH02_RS24040 (RBH02_24045) pilY1 5179509..5183000 (+) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  RBH02_RS24045 (RBH02_24050) pilY2 5183002..5183349 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  RBH02_RS24050 (RBH02_24055) comF 5183346..5183771 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  RBH02_RS24055 (RBH02_24060) ispH 5183818..5184762 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  RBH02_RS24060 (RBH02_24065) fkpB 5184848..5185288 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  RBH02_RS24065 (RBH02_24070) lspA 5185281..5185790 (-) 510 WP_003102615.1 signal peptidase II -
  RBH02_RS24070 (RBH02_24075) ileS 5185783..5188614 (-) 2832 WP_014603309.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=797762 RBH02_RS24050 WP_003094721.1 5183346..5183771(+) (comF) [Pseudomonas aeruginosa strain SRMPA3860]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=797762 RBH02_RS24050 WP_003094721.1 5183346..5183771(+) (comF) [Pseudomonas aeruginosa strain SRMPA3860]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383