Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   RBB89_RS26545 Genome accession   NZ_CP132949
Coordinates   5663248..5663673 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain IPA34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5658248..5668673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBB89_RS26530 (RBB89_26535) pilX 5658818..5659405 (+) 588 WP_033972921.1 type 4a pilus minor pilin PilX -
  RBB89_RS26535 (RBB89_26540) pilY1 5659417..5662902 (+) 3486 WP_088376618.1 type 4a pilus biogenesis protein PilY1 -
  RBB89_RS26540 (RBB89_26545) pilY2 5662904..5663251 (+) 348 WP_088376617.1 type 4a fimbrial biogenesis protein PilY2 -
  RBB89_RS26545 (RBB89_26550) comF 5663248..5663673 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  RBB89_RS26550 (RBB89_26555) ispH 5663720..5664664 (-) 945 WP_003134939.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  RBB89_RS26555 (RBB89_26560) fkpB 5664750..5665190 (-) 441 WP_088376616.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  RBB89_RS26560 (RBB89_26565) lspA 5665183..5665692 (-) 510 WP_025271359.1 signal peptidase II -
  RBB89_RS26565 (RBB89_26570) ileS 5665685..5668516 (-) 2832 WP_108240924.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=797430 RBB89_RS26545 WP_003094721.1 5663248..5663673(+) (comF) [Pseudomonas aeruginosa strain IPA34]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=797430 RBB89_RS26545 WP_003094721.1 5663248..5663673(+) (comF) [Pseudomonas aeruginosa strain IPA34]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTATTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383