Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   L6477_RS05915 Genome accession   NZ_AP022886
Coordinates   1152617..1156132 (+) Length   1171 a.a.
NCBI ID   WP_000058560.1    Uniprot ID   A0A7D3YSP4
Organism   Bacillus cereus strain MRY14-0074     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1147617..1161132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6477_RS05880 (BCM0074_1068) - 1147827..1148618 (-) 792 WP_236797619.1 phosphotransferase -
  L6477_RS05885 (BCM0074_1069) cspA 1148970..1149173 (+) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -
  L6477_RS05890 (BCM0074_1070) - 1149363..1149575 (-) 213 WP_236797621.1 hypothetical protein -
  L6477_RS05895 (BCM0074_1071) - 1149892..1150077 (-) 186 WP_000391382.1 hypothetical protein -
  L6477_RS05900 (BCM0074_1072) - 1150359..1150940 (+) 582 WP_236797623.1 competence protein ComK -
  L6477_RS05905 (BCM0074_1073) - 1151287..1151880 (+) 594 WP_000347516.1 TVP38/TMEM64 family protein -
  L6477_RS05910 (BCM0074_1074) lepB 1151937..1152500 (+) 564 WP_000751894.1 signal peptidase I -
  L6477_RS05915 (BCM0074_1075) addB 1152617..1156132 (+) 3516 WP_000058560.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  L6477_RS05920 (BCM0074_1076) addA 1156129..1159854 (+) 3726 WP_236797625.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  L6477_RS05925 (BCM0074_1077) - 1159867..1160154 (+) 288 WP_000718623.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  L6477_RS05930 (BCM0074_1078) gerPF 1160192..1160407 (-) 216 WP_001141566.1 spore germination protein GerPF -
  L6477_RS05935 (BCM0074_1079) - 1160450..1160836 (-) 387 WP_000902341.1 spore germination protein GerPE -
  L6477_RS05940 (BCM0074_1080) gerPD 1160852..1161046 (-) 195 WP_001052797.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134327.53 Da        Isoelectric Point: 5.3742

>NTDB_id=79742 L6477_RS05915 WP_000058560.1 1152617..1156132(+) (addB) [Bacillus cereus strain MRY14-0074]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVKGAEVYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEMHYEARPNEKFHGEASVTIHTAANL
RAEVEGVAREIRRLVAEENYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSTVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKSLDLTEVYYGLALQMLTYLDVVTSNAQTWMKKGHAASPAGVLYFHIHNPIVEVKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFKNIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=79742 L6477_RS05915 WP_000058560.1 1152617..1156132(+) (addB) [Bacillus cereus strain MRY14-0074]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAGGAAGAGTTAAA
ACAGCGTCCAAGAGGGGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGGTA
GTGAGGATGTTAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTATTGCAAGAAGTTGGT
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTGGGCAGTATGATTGCGGAGTTTAAACGATATA
ATGTGACGCCATCAAACGTATATGAAATGTGGCAACAATTAGATGCCCATAGTAGCAGTGCAGAACAAAAGCTACTGGCG
AATAAAGTGTATGATTTACAACTACTATATGATGATTTTGAGCGTGCTTTAATCGGAAAATATTTAGATTCAGAAGACTA
TCTGCAATTGCTAGTCGAAAAGCTTCCTCAATCTGAATATGTAAAGGGAGCTGAAGTTTATATAGATGGATTTCACTCAT
TTTCACCGCAAGAGTTAGAAATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAATTAGATTTATTTTATGAAACGACATTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACGATTCCACTTATGGAACAGCCGCGTTTTCATTCTCCGGCATTAGCGCATT
TAGAAATGCATTACGAAGCGCGTCCAAATGAAAAGTTTCACGGTGAAGCAAGTGTAACCATTCATACAGCAGCTAATTTA
CGAGCGGAAGTAGAAGGCGTTGCTCGTGAAATTCGTAGACTTGTGGCTGAAGAAAACTATCGTTACCGAGATATTGCGGT
TCTTCTTCGTAACGGGGAAAGTTATTACGATGTAATGCGGACACTATTTACAGATTATAATATCCCGCACTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGTTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTCTTTCGCTGCGTGAAAACAGAGCTTTTATATCCACTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTATTGCTTAGCATACGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGTCGCT
ATCGTTCTCTTGACGATACGAACGGGATGATCACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGTGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGCGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
CTTATTTTTAGAAGAGCTGGACGTTCCAAAAAAATTAGAAGCATTACGTATTCGTGCAGAAGAGAGTGGGGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTTATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTCGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTGATTGCTAATATTGATCGTTCAAGATTATCAAATGTGAAAGCAACATTTGTTATTGGCGTGAATGAAGGTG
TCATTCCAGCAGCACCGATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGATGTTTTGAGCGCTGCAGGTATTGAACTC
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
TATTTCATGCCCGCTTGCAGATGAGGAAGGGAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAATGACGTAAATGATTTATCACGTTCGGAACAAATTTCATACGTAGCAACGCCG
GAAGTAACACTGTCATATGTTATGCAGCAACTTCAAACGTGGAAGCGATATGGATTTGAAGGGAATTTAGACTTTTGGTG
GGATGTATATAATTTCTACGTAACTTCGGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGTTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGCTGTAAGTAGAGATTTATACGGAGACAAAATAAAAGGAAGCGTTTCTCGTATGGAA
CTATTTAATCGTTGTGCGTACGCTCATTTCGCACAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTTAAACTTGATGC
GCCAGATATCGGGGAGCTTTTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAATCGTACTTGGGCAG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTACTGTAGTAATAGAAGAGATTGCACCGTTATTACAAAGGCAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCCATTATTCTACGTGAACA
TGCGAAGTCGAGCGGTTTCGTACCAGTTGATTTAGAAGTGCCATTTGGTATGGGCGGTACAGGATCACTTCCGCCGATGG
AATTCTCGTTACCAAATGGTGTAAAGATGGAAGTAGTCGGCCGTATTGACCGCGTTGATAAGGCGGAAGATGAAAACGGA
ACATTCCTCCGTATTATTGACTATAAATCAAGCTCAAAATCGTTAGACTTAACGGAAGTGTATTACGGATTGGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACAGACGTGGATGAAAAAAGGCCACGCAGCATCACCAGCTGGTG
TACTGTACTTCCACATTCATAACCCAATTGTTGAGGTGAAAGGTGACGCATCTGAAGCAGAAATTGAAAAGGAAATTTTA
AAGAAATTCAAAATGAAAGGGCTCGTACTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
TTCAGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGTTTTAGTGCGCGTTCAAGTATTGCCAGTGAACAAGAGTTTA
ACGTCCTGCAAAAATACGTACATCATACGTTTAAAAATATCGGAAAAGACATTACAGAGGGTGTTATTGATATTGCTCCA
TACAAAAAGGGGAATAAAGCAGCGTGTACGTTCTGTAACTTCAAATCAGTTTGTCAGTTCGATGAATCACTTGAAGATAA
TCAATTCCGTACGCTAAAAGATATGAAAGATAGTGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(304-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D3YSP4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.147

100

0.492

Multiple sequence alignment