Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RA171_RS11185 Genome accession   NZ_CP132728
Coordinates   2286439..2286906 (+) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli strain LZSFT34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2281439..2291906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA171_RS11140 (RA171_11115) rplP 2281549..2281959 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  RA171_RS11145 (RA171_11120) rpsC 2281972..2282673 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  RA171_RS11150 (RA171_11125) rplV 2282691..2283023 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  RA171_RS11155 (RA171_11130) rpsS 2283038..2283316 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  RA171_RS11160 (RA171_11135) rplB 2283333..2284154 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  RA171_RS11165 (RA171_11140) rplW 2284172..2284474 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  RA171_RS11170 (RA171_11145) rplD 2284471..2285076 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  RA171_RS11175 (RA171_11150) rplC 2285087..2285716 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  RA171_RS11180 (RA171_11155) rpsJ 2285749..2286060 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  RA171_RS11185 (RA171_11160) pilD 2286439..2286906 (+) 468 WP_000339836.1 prepilin peptidase Machinery gene
  RA171_RS11190 (RA171_11165) bfr 2286903..2287379 (-) 477 WP_000675515.1 bacterioferritin -
  RA171_RS11195 (RA171_11170) bfd 2287452..2287646 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  RA171_RS11200 (RA171_11175) tuf 2287829..2289013 (-) 1185 WP_000031783.1 elongation factor Tu -
  RA171_RS11205 (RA171_11180) fusA 2289084..2291198 (-) 2115 WP_000124700.1 elongation factor G -
  RA171_RS11210 (RA171_11185) rpsG 2291295..2291765 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=796977 RA171_RS11185 WP_000339836.1 2286439..2286906(+) (pilD) [Escherichia coli strain LZSFT34]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=796977 RA171_RS11185 WP_000339836.1 2286439..2286906(+) (pilD) [Escherichia coli strain LZSFT34]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361