Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   PWG69_RS13385 Genome accession   NZ_CP118744
Coordinates   2688732..2689766 (-) Length   344 a.a.
NCBI ID   WP_026579310.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain RP01     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2683732..2694766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWG69_RS13335 (PWG69_13335) tasA 2683803..2684597 (-) 795 WP_020452167.1 biofilm matrix protein TasA -
  PWG69_RS13340 (PWG69_13340) sipW 2684670..2685254 (-) 585 WP_023855186.1 signal peptidase I SipW -
  PWG69_RS13345 (PWG69_13345) tapA 2685251..2685979 (-) 729 WP_020452169.1 amyloid fiber anchoring/assembly protein TapA -
  PWG69_RS13350 (PWG69_13350) - 2686257..2686577 (+) 321 WP_023855188.1 YqzG/YhdC family protein -
  PWG69_RS13355 (PWG69_13355) - 2686607..2686789 (-) 183 WP_025811164.1 YqzE family protein -
  PWG69_RS13360 (PWG69_13360) comGG 2686877..2687242 (-) 366 WP_026579313.1 competence type IV pilus minor pilin ComGG -
  PWG69_RS13365 (PWG69_13365) comGF 2687254..2687742 (-) 489 WP_229078638.1 competence type IV pilus minor pilin ComGF -
  PWG69_RS13370 (PWG69_13370) comGE 2687651..2687998 (-) 348 WP_025811161.1 competence type IV pilus minor pilin ComGE -
  PWG69_RS13375 (PWG69_13375) comGD 2687982..2688419 (-) 438 WP_026579312.1 competence type IV pilus minor pilin ComGD -
  PWG69_RS13380 (PWG69_13380) comGC 2688425..2688718 (-) 294 WP_026579311.1 competence type IV pilus major pilin ComGC Machinery gene
  PWG69_RS13385 (PWG69_13385) comGB 2688732..2689766 (-) 1035 WP_026579310.1 competence type IV pilus assembly protein ComGB Machinery gene
  PWG69_RS13390 (PWG69_13390) comGA 2689756..2690820 (-) 1065 WP_080131442.1 competence type IV pilus ATPase ComGA Machinery gene
  PWG69_RS13395 (PWG69_13395) - 2690978..2691817 (-) 840 WP_020452179.1 STAS domain-containing protein -
  PWG69_RS13405 (PWG69_13405) - 2692059..2692436 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  PWG69_RS13410 (PWG69_13410) - 2692647..2692892 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  PWG69_RS13415 (PWG69_13415) - 2692928..2693566 (-) 639 WP_020452181.1 MBL fold metallo-hydrolase -
  PWG69_RS13420 (PWG69_13420) - 2693722..2693895 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  PWG69_RS13425 (PWG69_13425) - 2693956..2694270 (-) 315 WP_020452182.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39730.11 Da        Isoelectric Point: 9.8784

>NTDB_id=794900 PWG69_RS13385 WP_026579310.1 2688732..2689766(-) (comGB) [Bacillus paralicheniformis strain RP01]
MKIKMKWPVREQADLLKKLGDMMKSGYTILDALSMLKLQLNERRRADLTFAMEKLTEGRPVFQVLEMISFHKDAVTIVYF
AEQHGNLPYAFRQSGELLHRKIAQTEKLKKAARYPLFLVLTVCVIISIMKSAIIPQFSAIYESMNVETSFATTLIFSVFD
HFYLFIAGMLLIAAALSLYYLCSFRHKPPEGKMTFLIRIPLLGQTFKLFNSYFLSLQLSNLLQAGLSVYDSLKAFESQPF
LSFHKNEAKRLIKRLKQGESLEQMLAGHPFYENDLAKAVAHGQLNGHLYRELYSYSQFLIERLERKAEKWTGLIQPLIYG
LTAAMILILYLSMLLPMYQMMNQL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=794900 PWG69_RS13385 WP_026579310.1 2688732..2689766(-) (comGB) [Bacillus paralicheniformis strain RP01]
ATGAAGATTAAAATGAAGTGGCCCGTCCGGGAACAGGCGGACCTTCTGAAAAAACTCGGAGACATGATGAAGAGCGGATA
TACGATTTTGGATGCTTTAAGTATGCTGAAACTGCAATTGAATGAAAGGCGAAGAGCAGATCTGACATTTGCGATGGAGA
AGCTGACAGAAGGGCGACCTGTGTTTCAAGTGCTTGAAATGATTTCATTTCATAAGGATGCTGTTACCATTGTTTACTTT
GCCGAACAGCACGGGAATTTGCCATATGCCTTTCGGCAGAGCGGAGAGCTGCTTCACCGCAAGATCGCTCAAACCGAAAA
ACTAAAGAAAGCCGCCAGATATCCCTTGTTTTTAGTTTTAACGGTCTGTGTCATTATCTCTATTATGAAATCGGCAATCA
TCCCGCAATTTTCCGCGATCTATGAATCAATGAACGTTGAAACGTCTTTTGCAACGACCCTCATCTTTTCGGTTTTTGAT
CATTTTTATCTTTTTATCGCAGGAATGCTGTTGATTGCCGCCGCTTTATCCCTCTATTATCTGTGTTCATTTCGCCATAA
ACCTCCAGAAGGCAAAATGACCTTTCTCATTAGAATTCCCTTGCTGGGACAAACCTTCAAACTATTCAACAGTTATTTTT
TATCGCTCCAATTGAGCAATCTTTTGCAAGCGGGTCTATCTGTATATGACAGTTTAAAAGCATTTGAAAGCCAGCCGTTT
TTATCATTTCATAAAAATGAAGCGAAGCGCCTGATCAAGCGGCTGAAACAAGGGGAGAGTCTGGAGCAGATGCTGGCCGG
GCATCCGTTTTATGAAAATGATCTGGCGAAGGCTGTTGCTCACGGCCAATTAAACGGACACTTATACAGAGAGCTGTATT
CATACAGTCAATTTTTGATTGAACGGCTTGAACGGAAAGCAGAAAAATGGACAGGTTTGATCCAGCCATTGATTTACGGG
CTAACAGCGGCGATGATATTAATTCTTTATTTGTCTATGCTTTTGCCAATGTATCAAATGATGAACCAGTTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(216-336)

Type II secretion system (T2SS), protein F (T2SSF)

(16-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

56.966

93.895

0.535