Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SKTS_RS18600 Genome accession   NZ_AP022853
Coordinates   3799678..3800736 (-) Length   352 a.a.
NCBI ID   WP_173068684.1    Uniprot ID   A0A6F8VJ18
Organism   Sulfurimicrobium lacus strain skT11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3794678..3805736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SKTS_RS18580 (SKTS_36470) - 3795204..3795524 (-) 321 WP_173068675.1 DUF485 domain-containing protein -
  SKTS_RS18585 (SKTS_36480) - 3795646..3796320 (+) 675 WP_173068678.1 response regulator transcription factor -
  SKTS_RS18590 (SKTS_36490) - 3796353..3797750 (+) 1398 WP_173068680.1 sensor histidine kinase -
  SKTS_RS18595 (SKTS_36500) - 3797747..3799648 (-) 1902 WP_173068682.1 DUF294 nucleotidyltransferase-like domain-containing protein -
  SKTS_RS18600 (SKTS_36510) pilU 3799678..3800736 (-) 1059 WP_173068684.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SKTS_RS18610 (SKTS_36520) - 3801118..3802116 (+) 999 WP_173068686.1 FAD:protein FMN transferase -
  SKTS_RS18615 (SKTS_36530) - 3802119..3802622 (+) 504 WP_244617395.1 SoxR reducing system RseC family protein -
  SKTS_RS18620 (SKTS_36540) rsxA 3802633..3803214 (+) 582 WP_173068688.1 electron transport complex subunit RsxA -
  SKTS_RS18625 (SKTS_36550) - 3803226..3803765 (+) 540 WP_173068689.1 RnfABCDGE type electron transport complex subunit B -
  SKTS_RS18630 (SKTS_36560) rsxC 3803775..3805250 (+) 1476 WP_173068691.1 electron transport complex subunit RsxC -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38865.67 Da        Isoelectric Point: 6.5096

>NTDB_id=79485 SKTS_RS18600 WP_173068684.1 3799678..3800736(-) (pilU) [Sulfurimicrobium lacus strain skT11]
MSDTRSLLQLMVDKGASDLLLTAETPPHIKIDGVTSPVDMPALKAGESKALAYAFMTKRQSKEFEETKESNFAVSVDGIG
RFRLNVYYQRGEVSVAIRQIKSTIPSFDELGLPQQARELSLLRRGLVLMVGAAGTGKSTTMAAMVDYRAKHMDGHILTIE
DPIEYCFAHGRSVVEQREVGLDTLSFGDALRNAMREAPDVIVIGEIRDRETAQHAIMYAEAGVLCISTMHSNNANQAIER
IINYFPEDIRRQVLLDLSLNLKGVIAQRLLPGTNGKQALATGVLLQSAYVSELIQNGKVDVLKEAMKKGIEEGMQVFDES
LFKLYKGGRISYEDALRNADSRTDLALRIKLS

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=79485 SKTS_RS18600 WP_173068684.1 3799678..3800736(-) (pilU) [Sulfurimicrobium lacus strain skT11]
ATGAGCGATACGAGAAGCCTTTTGCAGCTGATGGTGGACAAAGGTGCGTCCGATCTGCTTTTGACGGCCGAGACCCCCCC
GCATATCAAGATCGATGGAGTAACCAGCCCGGTCGATATGCCCGCGCTCAAAGCGGGCGAGTCGAAAGCGCTGGCGTACG
CCTTCATGACCAAGCGCCAGAGCAAGGAGTTCGAGGAGACCAAGGAATCCAACTTTGCGGTGTCGGTCGACGGCATTGGC
CGTTTCCGCCTGAACGTCTACTACCAGCGCGGCGAAGTGTCGGTGGCCATACGCCAGATCAAATCGACCATTCCGTCGTT
TGACGAACTGGGCTTGCCGCAGCAGGCGCGCGAGCTTTCCTTGCTCAGGCGAGGGCTGGTGCTGATGGTGGGGGCAGCCG
GGACCGGGAAAAGCACAACCATGGCTGCGATGGTCGATTACCGTGCCAAGCACATGGATGGGCATATCCTGACCATCGAG
GACCCGATCGAATATTGTTTCGCGCATGGCAGGTCGGTGGTCGAGCAGCGCGAGGTCGGGCTGGACACGCTGTCGTTCGG
GGATGCCCTGCGCAACGCGATGCGCGAGGCACCGGACGTCATCGTCATCGGCGAGATCCGAGATCGGGAGACCGCGCAGC
ATGCGATCATGTACGCGGAAGCCGGCGTGCTGTGCATCTCCACCATGCACTCCAACAACGCGAATCAGGCGATTGAGCGC
ATTATCAATTATTTCCCCGAAGATATCCGTCGTCAGGTCTTGCTCGATTTGTCTCTCAATCTGAAGGGTGTGATTGCACA
GCGCTTGTTGCCCGGCACCAACGGCAAGCAGGCGCTGGCAACGGGGGTGCTTCTGCAGTCGGCTTATGTTTCCGAACTGA
TCCAGAACGGCAAGGTTGACGTATTGAAAGAGGCGATGAAGAAAGGCATCGAAGAAGGCATGCAGGTTTTCGACGAAAGC
CTCTTCAAGCTTTATAAAGGTGGGCGGATCAGCTATGAAGATGCGTTGCGCAACGCGGATTCGCGCACCGATCTGGCGCT
GCGCATCAAGCTGTCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6F8VJ18

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.289

98.295

0.494
  pilU Vibrio cholerae strain A1552

49.56

96.875

0.48
  pilU Acinetobacter baylyi ADP1

46.023

100

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.012

96.023

0.403
  pilT Pseudomonas aeruginosa PAK

39.466

95.739

0.378
  pilT Acinetobacter baylyi ADP1

39.339

94.602

0.372
  pilT Pseudomonas stutzeri DSM 10701

38.576

95.739

0.369
  pilT Legionella pneumophila strain Lp02

38.739

94.602

0.366
  pilT Legionella pneumophila strain ERS1305867

38.739

94.602

0.366
  pilT Acinetobacter nosocomialis M2

38.138

94.602

0.361
  pilT Acinetobacter baumannii D1279779

38.138

94.602

0.361
  pilT Acinetobacter baumannii strain A118

38.138

94.602

0.361

Multiple sequence alignment