Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYH   Type   Machinery gene
Locus tag   PWF74_RS08330 Genome accession   NZ_CP118627
Coordinates   1663564..1664499 (-) Length   311 a.a.
NCBI ID   WP_274978345.1    Uniprot ID   A0AAX3NAR6
Organism   Lactococcus garvieae strain Pa-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1658564..1669499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWF74_RS08305 (PWF74_08305) - 1658611..1659471 (-) 861 WP_003133325.1 CvfB family protein -
  PWF74_RS08310 (PWF74_08310) frr 1659579..1660136 (-) 558 WP_003133324.1 ribosome recycling factor -
  PWF74_RS08315 (PWF74_08315) pyrH 1660166..1660882 (-) 717 WP_003133322.1 UMP kinase -
  PWF74_RS08320 (PWF74_08320) - 1660973..1662166 (-) 1194 WP_003133320.1 acetate kinase -
  PWF74_RS08325 (PWF74_08325) - 1662286..1663479 (-) 1194 WP_003133318.1 acetate kinase -
  PWF74_RS08330 (PWF74_08330) comYH 1663564..1664499 (-) 936 WP_274978345.1 class I SAM-dependent methyltransferase Machinery gene
  PWF74_RS08335 (PWF74_08335) - 1664545..1664775 (-) 231 WP_003133314.1 DUF3165 family protein -
  PWF74_RS08340 (PWF74_08340) typA 1664851..1666692 (-) 1842 WP_003133313.1 translational GTPase TypA -
  PWF74_RS08345 (PWF74_08345) - 1667013..1667225 (+) 213 WP_003133311.1 YqgQ family protein -
  PWF74_RS08350 (PWF74_08350) - 1667218..1668183 (+) 966 WP_003133309.1 ROK family glucokinase -
  PWF74_RS08355 (PWF74_08355) - 1668203..1668583 (+) 381 WP_003133308.1 rhodanese-like domain-containing protein -
  PWF74_RS08360 (PWF74_08360) - 1668623..1669069 (-) 447 WP_003133306.1 Dps family protein -

Sequence

Protein


Download         Length: 311 a.a.        Molecular weight: 35150.25 Da        Isoelectric Point: 4.6737

>NTDB_id=794060 PWF74_RS08330 WP_274978345.1 1663564..1664499(-) (comYH) [Lactococcus garvieae strain Pa-2]
MDIEKIEKAFGLVLENIMAIQAELLTDFYDAFVEQNAAYLGALEFASLTKENNDKVRSMNLTKSEWQKLFQFVLLQGSRV
APMQANHNLTPDSIGFIFNFIIEELHKERKIRVLEFGSGTGNLAETLLVHMQKEVDYVGFEVDDLLLDLSASMSEVMGTE
ASFLQIDAVKPQPLESVDVVMSDLPVGYYPDDETARHFQVHDKSEHTYVHHLMIEQSFKYLKESGFALFLAPDNLLTSAQ
SEFLKAWIKEHAHVAAVITLPSSLFKGAGKSIYLLSKNQINTPTFVYPLADLADRSILQEFMSEFVKNVKI

Nucleotide


Download         Length: 936 bp        

>NTDB_id=794060 PWF74_RS08330 WP_274978345.1 1663564..1664499(-) (comYH) [Lactococcus garvieae strain Pa-2]
ATGGATATAGAGAAAATCGAAAAAGCCTTTGGATTGGTTCTTGAAAATATTATGGCAATTCAAGCGGAGCTCTTGACGGA
TTTTTATGATGCTTTTGTGGAACAAAATGCAGCTTACTTAGGCGCTTTAGAGTTTGCATCTCTGACGAAGGAAAATAATG
ATAAGGTTCGTTCAATGAACCTCACGAAAAGTGAGTGGCAAAAGCTGTTTCAGTTTGTCTTGCTTCAAGGGTCACGTGTC
GCACCAATGCAAGCAAATCATAATTTAACACCAGATTCTATTGGGTTTATCTTTAATTTTATCATTGAAGAGTTGCACAA
AGAAAGAAAGATTCGTGTACTAGAATTTGGCTCAGGAACAGGGAATTTAGCGGAAACGCTCTTGGTTCACATGCAAAAAG
AAGTAGACTATGTTGGTTTTGAAGTAGATGACCTCTTGTTGGATTTGTCCGCTTCAATGTCCGAAGTCATGGGAACAGAA
GCAAGTTTTCTCCAAATTGACGCCGTGAAACCACAGCCCTTGGAGTCTGTAGATGTTGTGATGAGTGATCTCCCGGTTGG
TTATTATCCAGACGATGAAACTGCTCGCCATTTTCAAGTGCATGACAAATCAGAACATACCTATGTGCACCACTTAATGA
TTGAGCAATCCTTTAAGTATCTGAAGGAGAGTGGTTTTGCGCTCTTCTTAGCTCCAGATAATCTTTTGACAAGTGCACAG
TCAGAATTTTTGAAGGCTTGGATTAAAGAACATGCACATGTTGCTGCAGTCATTACATTGCCTTCATCACTCTTTAAAGG
TGCAGGAAAATCGATTTATTTATTAAGTAAAAACCAAATAAATACACCAACTTTTGTTTACCCCTTGGCTGATTTAGCAG
ACCGTTCTATTTTGCAAGAATTTATGTCTGAGTTTGTGAAAAATGTCAAGATTTGA

Domains

Predicted by InterproScan.

N-6 DNA Methylase (N6_Mtase)

(132-285)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYH Streptococcus mutans UA140

49.677

99.678

0.495
  comYH Streptococcus mutans UA159

49.355

99.678

0.492