Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   PWW22_RS16260 Genome accession   NZ_CP118578
Coordinates   3532803..3533528 (-) Length   241 a.a.
NCBI ID   WP_033007443.1    Uniprot ID   A0A8B3DV78
Organism   Vibrio harveyi strain fish2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 3531379..3552560 3532803..3533528 within 0


Gene organization within MGE regions


Location: 3531379..3552560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW22_RS16250 nudE 3531379..3531945 (-) 567 WP_009698975.1 ADP compounds hydrolase NudE -
  PWW22_RS16255 nfuA 3532121..3532705 (-) 585 WP_017189962.1 Fe-S biogenesis protein NfuA -
  PWW22_RS16260 comF 3532803..3533528 (-) 726 WP_033007443.1 ComF family protein Machinery gene
  PWW22_RS16265 bioH 3533613..3534377 (+) 765 WP_005448476.1 pimeloyl-ACP methyl ester esterase BioH -
  PWW22_RS16270 - 3534507..3534971 (+) 465 WP_009698973.1 hypothetical protein -
  PWW22_RS16275 - 3535076..3537397 (-) 2322 WP_017189965.1 Tex family protein -
  PWW22_RS16280 - 3537607..3539490 (+) 1884 WP_009698971.1 RimK/LysX family protein -
  PWW22_RS16285 - 3539563..3539907 (-) 345 Protein_3100 GreA/GreB family elongation factor -
  PWW22_RS16290 gmtY 3540250..3541545 (+) 1296 WP_274880594.1 gamma-mobile-trio recombinase GmtY -
  PWW22_RS16295 - 3541545..3545348 (+) 3804 WP_274880595.1 VPA1269 family protein -
  PWW22_RS16300 gmtX 3545350..3545973 (+) 624 WP_208445583.1 gamma-mobile-trio protein GmtX -
  PWW22_RS16305 greB 3545992..3546471 (+) 480 WP_274880597.1 transcription elongation factor GreB -
  PWW22_RS16310 - 3546468..3546782 (-) 315 WP_274880598.1 helix-turn-helix transcriptional regulator -
  PWW22_RS16315 - 3546885..3547415 (+) 531 WP_274880599.1 hypothetical protein -
  PWW22_RS16320 - 3547409..3548785 (+) 1377 WP_274880600.1 DEAD/DEAH box helicase family protein -
  PWW22_RS16325 - 3548872..3550977 (+) 2106 WP_274880601.1 type I restriction-modification system subunit M -
  PWW22_RS16330 - 3550992..3552560 (+) 1569 WP_274880602.1 type I restriction-modification system subunit M -

Sequence

Protein


Download         Length: 241 a.a.        Molecular weight: 27681.74 Da        Isoelectric Point: 8.4332

>NTDB_id=793540 PWW22_RS16260 WP_033007443.1 3532803..3533528(-) (comF) [Vibrio harveyi strain fish2]
MLSHQWQNIMHRVLSSQCGLCRFPIPHRQTPNLMRWCDSCFDHLTPIKRCSRCGLKMTEEESQSSAECGECLKEPPPWRR
LYTLGDYDFPLSQQVQRFKDHGEAWQVSTLTQLLAERIEQPAPVITSVPVHWQRYIKRGFNQSHILTKHLARHLDVRYES
KIFKRVKSAQSQRGNKKASREQNLQGAFALQGEVDFSHVAIVDDVVTTGSTVRQLCHLLLEVGVESIDIYCICRTPAPGS
I

Nucleotide


Download         Length: 726 bp        

>NTDB_id=793540 PWW22_RS16260 WP_033007443.1 3532803..3533528(-) (comF) [Vibrio harveyi strain fish2]
ATGTTATCTCATCAATGGCAAAACATCATGCATCGCGTACTCAGTAGCCAATGCGGATTATGCCGATTTCCCATACCTCA
CCGCCAAACGCCCAACCTAATGCGTTGGTGTGACAGCTGTTTTGACCACCTCACACCAATCAAACGCTGCTCACGTTGTG
GTTTAAAAATGACAGAAGAGGAATCACAATCCTCCGCAGAGTGCGGAGAATGCTTGAAAGAGCCACCACCATGGCGGCGT
TTGTATACCTTAGGTGACTATGACTTTCCGCTCTCTCAACAAGTACAGCGCTTTAAAGATCACGGCGAGGCATGGCAGGT
GAGTACGCTTACTCAACTATTAGCAGAACGAATTGAGCAGCCTGCCCCCGTCATCACCAGCGTGCCCGTTCACTGGCAGC
GCTACATAAAACGAGGATTCAATCAGAGCCATATCTTAACCAAACACCTCGCCCGCCATTTAGATGTGCGATATGAAAGC
AAGATATTTAAACGTGTCAAAAGCGCTCAGTCGCAGCGAGGAAACAAAAAAGCCAGTCGCGAGCAAAATCTGCAAGGGGC
ATTTGCCCTACAAGGTGAGGTGGACTTTTCCCACGTCGCGATTGTGGATGATGTGGTTACAACAGGTAGTACGGTCCGAC
AATTATGTCATTTACTACTTGAAGTTGGCGTAGAAAGCATCGATATTTACTGCATCTGCAGAACTCCTGCCCCCGGCTCA
ATCTAG

Domains

Predicted by InterproScan.

Phosphoribosyl transferase domain (Pribosyltran)

(145-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8B3DV78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Vibrio campbellii strain DS40M4

91.701

100

0.917
  comF Vibrio cholerae strain A1552

47.479

98.755

0.469