Detailed information NTG0793119

Bioinformatically predicted

Overview

• Name: recD2
• Type: Machinery gene
• Locus tag: PWA59_RS14360
• Genome accession: NZ_CP118541
• Coordinates: 2855567..2857945 (-)
• Length: 792 a.a.
• NCBI ID: WP_014418522.1
• Uniprot ID: I2C8C6
• Organism: Bacillus velezensis strain HC-5
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2850567..2862945

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PWA59_RS14325 (PWA59_14325)	-	2851639..2852367 (+)	729	WP_014418519.1	amino acid ABC transporter ATP-binding protein	-
PWA59_RS14330 (PWA59_14330)	-	2852388..2853215 (+)	828	WP_007408208.1	transporter substrate-binding domain-containing protein	-
PWA59_RS14335 (PWA59_14335)	-	2853272..2853922 (+)	651	WP_014418520.1	amino acid ABC transporter permease	-
PWA59_RS14340 (PWA59_14340)	-	2853941..2854597 (+)	657	WP_014418521.1	amino acid ABC transporter permease	-
PWA59_RS14345 (PWA59_14345)	-	2854633..2854764 (-)	132	WP_003152741.1	YrzQ family protein	-
PWA59_RS14350 (PWA59_14350)	-	2854786..2854977 (-)	192	WP_003152738.1	hypothetical protein	-
PWA59_RS14355 (PWA59_14355)	-	2854990..2855448 (-)	459	WP_007408205.1	PRC-barrel domain-containing protein	-
PWA59_RS14360 (PWA59_14360)	recD2	2855567..2857945 (-)	2379	WP_014418522.1	ATP-dependent RecD-like DNA helicase	Machinery gene
PWA59_RS14365 (PWA59_14365)	-	2857964..2858614 (-)	651	WP_007408203.1	tetratricopeptide repeat protein	-
PWA59_RS14370 (PWA59_14370)	mnmA	2858672..2859787 (-)	1116	WP_012118077.1	tRNA 2-thiouridine(34) synthase MnmA	-
PWA59_RS14375 (PWA59_14375)	-	2859824..2860963 (-)	1140	WP_007612815.1	cysteine desulfurase family protein	-
PWA59_RS14380 (PWA59_14380)	cymR	2860982..2861398 (-)	417	WP_003152730.1	cysteine metabolism transcriptional regulator CymR	-
PWA59_RS14385 (PWA59_14385)	-	2861593..2862861 (+)	1269	WP_014418523.1	replication-associated recombination protein A	-

Sequence

Protein

• Length: 792 a.a.
• Molecular weight: 88412.10 Da
• Isoelectric Point: 4.9490

>NTDB_id=793119 PWA59_RS14360 WP_014418522.1 2855567..2857945(-) (recD2) [Bacillus velezensis strain HC-5]
MDQLNIEEQPYVKGVIHTVIYHNDTNLYTVLKVKVAETSEAIEDKVVSVTGYFPLLQEDETYTFYGKTATHPKFGLQFQA
EHFKKEIPTTKEGIIHYLSSDLFEGIGKKTAEEIVKKLGDGALNKILTDASVLYDVPKMSKKKADKLAGELQRHQGLEQI
MISLNQYGFGPQLSMKIYQAYETETLEKIQENPYQLVKDVEGIGFVKADELGGKMGLSGNHPERIKAAILYTIESTCLSE
GHTYIETKQLIINTQTLLNQSAEGGRVTEMDAADGIIALGESKDIVIEGERCYFPSLFYAEQNVAKRVLDIAAQTEYADQ
FPESEFLLALGELEERMNVQYAPSQKDAIQKALSSPMLLLTGGPGTGKTTVIKGIVELYSELHGVSLDPGDYKKDETFPV
LLAAPTGRAAKRMSESTGLPAVTIHRLLGWNGAEGFTYNEDQPIEGKLIIIDEASMLDIWLASHLFKALPKHIQLIVVGD
EDQLPSVGPGQVLRDLLASEVLPTVRLTDIYRQAEGSSIVELAHDMKKGLLPKGLTAPTKDRSFIRCNSSQMKEVVDQVV
SNALKKGYTSRDIQVLAPMYRGKAGINELNMLLQDIMNPPKEKRRELKFGDVVYRTGDKILQLVNQPENNVFNGDIGEIV
SIFYAKENTEKEDMVVVSFDGNEITFTKKDFSQFTHAYCCSIHKSQGSEFPIVVLPVVKGYYRMLRRNLLYTAITRAKKF
LILCGEEEALEWGVKNNDATVRQTSLKNRLTEQTEEMDAELAALQKELPFSVYDANIGMEGITPFDFMEEKA

Nucleotide

• Length: 2379 bp

>NTDB_id=793119 PWA59_RS14360 WP_014418522.1 2855567..2857945(-) (recD2) [Bacillus velezensis strain HC-5]
GTGGATCAGCTGAACATCGAAGAACAGCCGTATGTAAAAGGCGTCATTCATACGGTCATTTATCATAATGACACCAACCT
GTATACAGTGCTGAAAGTCAAAGTTGCAGAAACGTCTGAGGCGATCGAAGACAAGGTCGTATCCGTAACGGGCTACTTCC
CTCTGCTGCAAGAAGATGAGACCTACACATTTTATGGAAAAACCGCCACGCATCCTAAGTTCGGCCTGCAATTTCAGGCT
GAACACTTTAAAAAAGAAATTCCGACCACAAAAGAAGGGATCATTCACTATTTATCAAGTGATCTGTTTGAAGGAATCGG
AAAAAAAACGGCGGAAGAAATCGTCAAAAAGCTCGGAGACGGCGCGCTCAATAAAATTCTGACGGATGCGTCGGTGCTTT
ACGATGTGCCGAAAATGTCGAAAAAGAAAGCGGATAAGCTTGCCGGAGAGCTGCAGCGGCATCAGGGGCTTGAGCAGATT
ATGATCTCTCTGAACCAATACGGTTTCGGGCCCCAGCTATCAATGAAAATATATCAGGCCTACGAAACGGAAACCCTGGA
GAAAATTCAGGAAAATCCTTATCAGCTTGTAAAAGACGTTGAAGGCATCGGCTTTGTGAAAGCTGACGAGCTGGGCGGAA
AAATGGGTCTGTCAGGAAACCACCCGGAGCGGATCAAAGCGGCAATCTTATATACGATTGAATCGACCTGTCTTTCAGAA
GGCCATACGTACATAGAGACAAAGCAGCTGATTATCAATACGCAAACGCTCCTGAATCAGTCAGCTGAAGGCGGCCGCGT
CACGGAGATGGATGCGGCTGACGGCATCATCGCCCTCGGGGAGAGTAAGGATATCGTCATAGAAGGAGAGCGCTGCTATT
TTCCGTCCTTGTTTTACGCCGAACAAAATGTCGCAAAACGCGTGCTCGACATTGCGGCTCAGACGGAGTATGCCGATCAA
TTTCCTGAATCGGAATTTCTGCTTGCACTCGGTGAACTGGAAGAACGGATGAATGTTCAATACGCACCAAGCCAAAAGGA
TGCCATTCAAAAAGCGCTGTCTTCACCGATGCTTTTATTGACCGGAGGACCCGGGACGGGGAAAACGACCGTTATTAAAG
GAATCGTGGAATTATACAGTGAACTGCACGGAGTATCGCTTGACCCCGGTGATTATAAAAAAGACGAGACGTTCCCGGTG
CTGCTTGCCGCGCCGACGGGAAGAGCCGCAAAACGGATGAGCGAATCTACGGGGCTGCCGGCTGTTACGATTCACAGGCT
GCTCGGCTGGAATGGCGCTGAAGGCTTTACCTACAATGAAGATCAGCCGATTGAAGGGAAACTGATCATTATAGACGAAG
CGAGTATGCTCGATATCTGGCTCGCCAGCCATTTGTTTAAGGCGCTTCCTAAGCACATTCAGCTCATTGTCGTCGGTGAT
GAAGACCAGCTTCCTTCAGTAGGACCGGGGCAGGTGCTCAGAGACCTTTTGGCTTCTGAGGTGCTGCCGACCGTGCGTTT
GACAGATATTTACCGTCAGGCTGAAGGATCATCCATTGTGGAACTTGCGCATGATATGAAAAAAGGACTGCTTCCAAAAG
GGCTGACCGCGCCGACAAAGGACCGCTCATTTATCCGCTGTAATTCTTCACAGATGAAAGAAGTCGTGGATCAGGTCGTC
TCGAACGCGTTAAAAAAGGGGTATACCTCCAGGGATATTCAAGTGCTTGCCCCGATGTACAGGGGAAAAGCGGGAATTAA
CGAGCTGAATATGCTTCTTCAAGACATCATGAATCCGCCGAAAGAAAAAAGGCGGGAGCTGAAATTCGGTGATGTCGTCT
ACAGGACGGGCGATAAAATATTACAGCTTGTCAACCAGCCGGAAAACAATGTGTTTAATGGCGATATCGGCGAAATCGTG
TCCATTTTTTATGCCAAAGAAAATACGGAAAAAGAAGACATGGTCGTCGTCTCGTTTGACGGCAACGAAATCACCTTTAC
GAAAAAGGATTTCAGCCAGTTTACCCACGCTTACTGCTGTTCCATTCATAAGTCGCAGGGAAGCGAATTCCCGATTGTCG
TCCTGCCTGTCGTCAAAGGCTACTATCGAATGCTCAGGAGAAATCTGCTTTATACCGCCATAACAAGGGCGAAAAAATTT
CTGATTCTTTGCGGAGAAGAAGAAGCGCTTGAATGGGGCGTCAAAAACAATGATGCCACCGTCAGACAGACGTCGCTTAA
AAACAGGCTCACAGAGCAGACTGAAGAGATGGATGCAGAACTGGCCGCCCTGCAAAAAGAGCTGCCGTTCAGTGTATATG
ATGCGAACATCGGAATGGAAGGCATCACGCCGTTTGACTTTATGGAAGAAAAAGCGTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix containing domain (HHH_RecD2) (154-244)

• AAA domain (AAA_30) (341-548)

• ATP-dependent RecD-like DNA helicase SH3 domain (SH3_13) (589-659)

• UvrD-like helicase C-terminal domain (UvrD_C_2) (676-724)

3D structure

• AlphaFold DB: I2C8C6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recD2	Bacillus subtilis subsp. subtilis str. 168	88.622	99.874	0.885