Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PWH45_RS16110 Genome accession   NZ_CP118383
Coordinates   3378425..3379405 (-) Length   326 a.a.
NCBI ID   WP_032619064.1    Uniprot ID   A0AA37AL66
Organism   Enterobacter hormaechei strain 2020CK-00224     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3373425..3384405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWH45_RS16080 (PWH45_16080) - 3374303..3374800 (+) 498 WP_047746945.1 SprT family zinc-dependent metalloprotease -
  PWH45_RS16085 (PWH45_16085) endA 3374895..3375602 (+) 708 WP_047746947.1 deoxyribonuclease I -
  PWH45_RS16090 (PWH45_16090) rsmE 3375654..3376385 (+) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PWH45_RS16095 (PWH45_16095) gshB 3376417..3377364 (+) 948 WP_015571835.1 glutathione synthase -
  PWH45_RS16100 (PWH45_16100) - 3377439..3377999 (+) 561 WP_006811924.1 YqgE/AlgH family protein -
  PWH45_RS16105 (PWH45_16105) ruvX 3377999..3378415 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  PWH45_RS16110 (PWH45_16110) pilT 3378425..3379405 (-) 981 WP_032619064.1 type IV pilus twitching motility protein PilT Machinery gene
  PWH45_RS16115 (PWH45_16115) - 3379423..3380124 (+) 702 WP_047746949.1 YggS family pyridoxal phosphate-dependent enzyme -
  PWH45_RS16120 (PWH45_16120) - 3380146..3380712 (+) 567 WP_015571838.1 YggT family protein -
  PWH45_RS16125 (PWH45_16125) yggU 3380709..3381005 (+) 297 WP_003860023.1 DUF167 family protein YggU -
  PWH45_RS16130 (PWH45_16130) - 3381009..3381602 (+) 594 WP_015571839.1 XTP/dITP diphosphatase -
  PWH45_RS16135 (PWH45_16135) hemW 3381595..3382743 (+) 1149 WP_017382926.1 radical SAM family heme chaperone HemW -
  PWH45_RS16140 (PWH45_16140) - 3382800..3383138 (+) 339 WP_047746950.1 endonuclease domain-containing protein -
  PWH45_RS16145 (PWH45_16145) - 3383190..3383906 (-) 717 WP_003862419.1 DUF2884 domain-containing protein -
  PWH45_RS16150 (PWH45_16150) - 3383964..3384290 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35706.68 Da        Isoelectric Point: 6.4131

>NTDB_id=792342 PWH45_RS16110 WP_032619064.1 3378425..3379405(-) (pilT) [Enterobacter hormaechei strain 2020CK-00224]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=792342 PWH45_RS16110 WP_032619064.1 3378425..3379405(-) (pilT) [Enterobacter hormaechei strain 2020CK-00224]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAAGCATTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAGCTGCTGTCCAATGACAATGGCCTGATTCTGGTCACTGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCATACGCGCGGCGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCAGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374