Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   PUW70_RS03475 Genome accession   NZ_CP118029
Coordinates   685339..686184 (+) Length   281 a.a.
NCBI ID   WP_024051891.1    Uniprot ID   A0A412PPI9
Organism   Streptococcus anginosus strain VSI68     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 680339..691184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW70_RS03445 (PUW70_03445) - 680562..681440 (+) 879 WP_024051896.1 F0F1 ATP synthase subunit gamma -
  PUW70_RS03450 (PUW70_03450) atpD 681475..682881 (+) 1407 WP_024051895.1 F0F1 ATP synthase subunit beta -
  PUW70_RS03455 (PUW70_03455) - 682892..683311 (+) 420 WP_003069748.1 F0F1 ATP synthase subunit epsilon -
  PUW70_RS03460 (PUW70_03460) - 683415..683645 (+) 231 WP_083308049.1 DUF1146 family protein -
  PUW70_RS03465 (PUW70_03465) murA 683683..684963 (+) 1281 WP_024051893.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  PUW70_RS03470 (PUW70_03470) - 684956..685132 (+) 177 WP_024051892.1 DNA-directed RNA polymerase subunit beta -
  PUW70_RS03475 (PUW70_03475) endA 685339..686184 (+) 846 WP_024051891.1 DNA/RNA non-specific endonuclease Machinery gene
  PUW70_RS03480 (PUW70_03480) - 686595..687739 (-) 1145 Protein_647 IS110 family transposase -
  PUW70_RS03485 (PUW70_03485) - 687991..689727 (+) 1737 WP_024051888.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 281 a.a.        Molecular weight: 30753.54 Da        Isoelectric Point: 10.4823

>NTDB_id=789243 PUW70_RS03475 WP_024051891.1 685339..686184(+) (endA) [Streptococcus anginosus strain VSI68]
MVRKKSNQKVTQSVAGLVIALVLALGGYSFSNHHSSPKPSDSTAINRSIRTNHAAPSQELAQSVVIESVKRQLKGKIEWN
GAGAFIVNENKNDLDAKVGSIPYADNKTKLVRGRTVPTVANALLSKATRQYRNREETGNGSTTWTPAGWHQVKHLSGEYH
HAVDRGHLLGYALIGNLKGFDASTSNPKNIALQTAWANQANTGHSTGQNFYETKVRKALDKNKRVRYRVTLIYANEQDLV
PVGSHIEAKSSDSSLEINVFVPNVQTGLRLNYQTGEVTVTN

Nucleotide


Download         Length: 846 bp        

>NTDB_id=789243 PUW70_RS03475 WP_024051891.1 685339..686184(+) (endA) [Streptococcus anginosus strain VSI68]
ATGGTGAGAAAGAAATCCAACCAGAAAGTAACACAAAGTGTAGCTGGTCTGGTTATAGCACTGGTTCTTGCGCTAGGAGG
TTATTCTTTTAGCAATCATCATAGTAGTCCGAAGCCTTCTGACAGCACTGCTATTAATCGAAGCATTCGGACGAATCATG
CAGCACCCAGTCAAGAATTAGCACAGAGCGTTGTAATAGAATCTGTCAAACGACAGCTCAAAGGGAAAATCGAGTGGAAT
GGCGCAGGGGCTTTTATTGTCAATGAGAATAAAAATGATTTAGATGCTAAAGTGGGCAGTATTCCTTATGCGGATAATAA
GACAAAATTGGTCAGAGGTCGAACTGTTCCGACGGTAGCAAATGCTCTTTTGTCTAAAGCTACTCGTCAGTATAGGAATC
GTGAAGAAACAGGTAATGGTTCAACAACTTGGACACCTGCTGGCTGGCATCAGGTGAAGCATTTATCGGGTGAATATCAT
CATGCAGTTGACCGTGGACATCTGCTAGGTTATGCCTTGATTGGTAACTTGAAAGGCTTTGATGCTTCGACTAGCAATCC
TAAAAATATTGCGCTGCAAACGGCTTGGGCCAATCAAGCAAATACTGGTCATTCTACGGGTCAAAATTTTTATGAAACAA
AGGTACGTAAGGCACTAGACAAAAATAAACGAGTTCGGTATCGGGTGACTTTAATTTATGCTAATGAACAGGATTTGGTG
CCGGTTGGTTCGCATATTGAAGCTAAATCAAGCGATAGTAGCTTGGAAATAAATGTCTTTGTTCCCAACGTACAGACAGG
ACTTCGGCTAAATTATCAAACAGGAGAAGTGACTGTTACCAACTAG

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(112-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A412PPI9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

68

97.865

0.665
  endA Streptococcus pneumoniae D39

68

97.865

0.665
  endA Streptococcus pneumoniae R6

68

97.865

0.665
  endA Streptococcus pneumoniae TIGR4

68

97.865

0.665