Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   PT671_RS16960 Genome accession   NZ_CP118021
Coordinates   3406054..3408414 (+) Length   786 a.a.
NCBI ID   WP_003222692.1    Uniprot ID   -
Organism   Bacillus spizizenii strain B-354     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3401054..3413414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT671_RS16940 comN 3402375..3402671 (+) 297 WP_003222684.1 post-transcriptional regulator ComN -
  PT671_RS16945 secDF 3402710..3404923 (+) 2214 WP_003222686.1 protein translocase subunit SecDF -
  PT671_RS16950 - 3405081..3405578 (+) 498 WP_003222688.1 cation:proton antiporter regulatory subunit -
  PT671_RS16955 - 3405655..3405987 (+) 333 WP_003222691.1 LapA family protein -
  PT671_RS16960 recJ 3406054..3408414 (+) 2361 WP_003222692.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  PT671_RS16965 - 3408420..3408932 (+) 513 WP_003222694.1 adenine phosphoribosyltransferase -
  PT671_RS16970 relA 3409101..3411305 (+) 2205 WP_003222696.1 GTP diphosphokinase -
  PT671_RS16975 dtd 3411318..3411758 (+) 441 WP_003222698.1 D-aminoacyl-tRNA deacylase -
  PT671_RS16980 - 3411783..3413336 (-) 1554 WP_003222700.1 SH3 domain-containing protein -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 88163.70 Da        Isoelectric Point: 4.9707

>NTDB_id=789177 PT671_RS16960 WP_003222692.1 3406054..3408414(+) (recJ) [Bacillus spizizenii strain B-354]
MLASKMRWEIQRPDQDKVKSLTEQLHITPLVASLLVKRGFDTAESARLFLHTKDADFYDPFEMKGMKEAVDRIKQAISQQ
EQIMIYGDYDADGVTSTSVMLHTLQKLSAQVDFYIPDRFKEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAKVAKEL
GLDVIITDHHEPGPELPNVHAIVHPKQPGCTYPFKELAGVGVAFKLAHALLGELPDELLDLAAIGTIADLVPLHDENRLI
ATLGLERLRRTNRLGLKELVKLSGGDIGEANEETVGFQIAPRLNAVGRIEQADPAVHLLMSEDSFEAEELAAEIDQLNKE
RQKMVSKMTDEAIEMVEQQGLDQTAIVVAKSGWNPGVVGIVASKLVDRFYRPAIVLGIDEEKGIAKGSARSIRGFNLFES
LSECRDILPHFGGHPMAAGMTLNAEDVPDLRNRLNEIADNTLTEEDFIPVQEVDLVCGVEDITVESITEMNLLSPFGMLN
PKPHVLVENAVLEDVRKIGANKNHVKMTVRNESSQLDCVGFNKGELEEGIVPGSRISIVGEMSINEWNNRKKPQLMIKDA
AVSEWQLFDLRGKRMWEDTVSVLPSAKRAIVSFKEDSTTLLQTEDLRREVHVISSEDQAKAFDLDGAYIVLLDPPPSLDM
LARLLEGKAPERIYFIFLNQEDHFLSTFPARDHFKWYYAFLLKRGAFDVKKHGSELARHKGWSVETINFMTKVFFDLGFV
KIENGVLSVVSGVKKRDLTDSQTYQAKQQLMELDQKLNYSSAEELKEWLNKLMKQDSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=789177 PT671_RS16960 WP_003222692.1 3406054..3408414(+) (recJ) [Bacillus spizizenii strain B-354]
ATGTTAGCGTCAAAAATGCGATGGGAAATCCAGCGACCAGATCAGGATAAGGTCAAATCACTCACAGAACAATTGCATAT
AACACCTCTTGTTGCGTCCCTGCTTGTAAAAAGAGGCTTTGATACGGCAGAAAGCGCAAGGCTGTTTTTACATACAAAGG
ATGCCGATTTTTATGATCCTTTCGAAATGAAGGGCATGAAAGAAGCGGTTGATCGTATAAAACAAGCGATTTCACAACAA
GAACAGATTATGATATATGGCGATTATGACGCCGATGGGGTTACCAGTACGTCAGTGATGCTTCACACACTGCAAAAGCT
GTCGGCTCAAGTTGATTTTTATATACCTGACCGCTTTAAAGAAGGATATGGCCCTAATGAACAGGCGTTTCGTTCGATTA
AAGAACGGGGCTTCTCTCTGATTATTACAGTTGATACAGGGATTGCCGCTGTACATGAAGCGAAGGTGGCAAAGGAACTT
GGGCTGGATGTCATTATTACCGATCACCATGAGCCGGGACCGGAGCTTCCGAATGTCCATGCGATCGTCCACCCGAAGCA
GCCTGGCTGCACCTACCCGTTTAAAGAACTTGCCGGAGTTGGGGTCGCGTTTAAGCTCGCCCACGCTTTATTGGGCGAAC
TCCCGGATGAACTGCTTGACTTGGCGGCAATCGGCACCATTGCCGATCTTGTCCCGTTGCATGACGAAAATAGATTAATT
GCAACACTAGGACTTGAAAGGCTTCGTCGGACGAATCGACTGGGCTTAAAAGAGCTGGTCAAGCTGTCAGGCGGAGACAT
CGGAGAAGCGAACGAAGAAACTGTCGGGTTCCAGATAGCACCAAGGCTGAATGCCGTCGGACGAATCGAACAAGCGGATC
CCGCTGTTCATTTGCTCATGTCAGAGGATTCGTTTGAAGCGGAAGAGCTTGCCGCTGAAATTGACCAGCTGAACAAAGAG
CGCCAAAAAATGGTCAGTAAAATGACGGATGAAGCGATTGAAATGGTTGAGCAGCAAGGTCTCGATCAAACTGCAATCGT
CGTGGCCAAATCCGGGTGGAATCCAGGAGTTGTGGGGATTGTGGCTTCAAAGCTTGTGGACCGTTTCTACCGTCCGGCCA
TTGTGCTTGGCATTGATGAAGAAAAAGGGATCGCTAAAGGCTCGGCCCGAAGCATTAGAGGCTTTAACTTATTCGAGAGC
CTTTCAGAGTGCAGAGATATTCTTCCTCATTTCGGGGGCCACCCAATGGCTGCGGGGATGACGCTGAATGCCGAAGATGT
GCCGGATTTGCGGAACAGGCTGAATGAAATAGCAGACAACACGCTTACGGAAGAAGATTTTATTCCTGTACAGGAAGTCG
ATCTTGTCTGCGGTGTCGAGGACATTACAGTTGAAAGCATCACAGAAATGAACCTGCTGTCCCCATTTGGCATGCTGAAC
CCAAAACCGCACGTTCTCGTAGAAAACGCTGTGCTGGAAGATGTCCGCAAGATCGGCGCTAATAAAAATCATGTGAAAAT
GACGGTCAGAAACGAGTCGTCTCAGCTTGATTGCGTCGGTTTTAATAAAGGTGAGCTCGAAGAAGGCATTGTCCCGGGTT
CAAGAATTTCAATTGTCGGTGAGATGTCTATCAACGAATGGAACAATAGAAAAAAACCGCAGCTAATGATTAAGGATGCC
GCTGTTTCTGAATGGCAGCTGTTTGATTTAAGGGGAAAACGGATGTGGGAAGACACAGTTTCCGTCCTTCCATCTGCTAA
ACGGGCCATTGTCTCTTTCAAAGAAGACTCAACAACACTTCTTCAGACTGAAGACCTTCGCCGTGAAGTGCACGTCATCA
GCTCTGAAGATCAAGCGAAGGCGTTTGACCTTGATGGTGCATATATTGTATTGCTGGATCCGCCGCCTTCGTTGGACATG
CTCGCACGTTTGCTGGAAGGCAAGGCGCCTGAGCGGATATATTTCATTTTTCTCAATCAAGAGGACCACTTTTTATCAAC
ATTTCCTGCAAGGGACCACTTTAAGTGGTATTATGCGTTCTTGCTGAAAAGAGGCGCTTTTGACGTAAAAAAACACGGTT
CAGAGCTTGCGAGACATAAAGGCTGGTCCGTTGAAACAATCAATTTCATGACAAAGGTGTTTTTTGATCTAGGTTTTGTT
AAAATAGAGAATGGTGTGCTGTCTGTGGTTAGCGGAGTAAAGAAACGCGACTTAACAGACTCACAAACATATCAGGCGAA
ACAGCAGCTGATGGAACTGGATCAAAAGCTGAATTATTCTTCTGCTGAAGAGCTGAAGGAATGGCTGAATAAGCTGATGA
AACAAGATTCAGAGGCTTATGAAAGTACGAGGAGGACATAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-437)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)

RecJ OB domain (RecJ_OB)

(453-559)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

97.455

100

0.975