Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   PT671_RS01285 Genome accession   NZ_CP118021
Coordinates   343377..344270 (-) Length   297 a.a.
NCBI ID   WP_003220839.1    Uniprot ID   E0TUA0
Organism   Bacillus spizizenii strain B-354     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 297164..366927 343377..344270 within 0


Gene organization within MGE regions


Location: 297164..366927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT671_RS01050 proS 299837..301531 (-) 1695 WP_003220935.1 proline--tRNA ligase -
  PT671_RS01055 rseP 301564..302832 (-) 1269 WP_079996361.1 RIP metalloprotease RseP -
  PT671_RS01060 dxr 302839..303990 (-) 1152 WP_003220931.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
  PT671_RS01065 cdsA 304052..304861 (-) 810 WP_079996360.1 phosphatidate cytidylyltransferase -
  PT671_RS01070 - 304865..305647 (-) 783 WP_003220927.1 isoprenyl transferase -
  PT671_RS01075 frr 305778..306335 (-) 558 WP_003220926.1 ribosome recycling factor -
  PT671_RS01080 pyrH 306337..307059 (-) 723 WP_003220923.1 UMP kinase -
  PT671_RS01085 tsf 307205..308086 (-) 882 WP_003220921.1 translation elongation factor Ts -
  PT671_RS01090 rpsB 308188..308928 (-) 741 WP_003220918.1 30S ribosomal protein S2 -
  PT671_RS01095 - 309072..309575 (-) 504 WP_003220913.1 hypothetical protein -
  PT671_RS01100 sigD 309604..310368 (-) 765 WP_003220911.1 RNA polymerase sigma-28 factor SigD -
  PT671_RS01105 cheD 310391..310891 (-) 501 WP_003220909.1 chemoreceptor glutamine deamidase CheD -
  PT671_RS01110 cheC 310888..311517 (-) 630 WP_003220907.1 CheY-P phosphatase CheC -
  PT671_RS01115 cheW 311536..312006 (-) 471 WP_003220905.1 chemotaxis protein CheW -
  PT671_RS01120 cheA 312028..314043 (-) 2016 WP_003220903.1 chemotaxis protein CheA -
  PT671_RS01125 cheB 314049..315119 (-) 1071 WP_003220901.1 protein-glutamate O-methylesterase CheB -
  PT671_RS01130 flhG 315121..316017 (-) 897 WP_079996359.1 flagellum location/number ATPase FlhG -
  PT671_RS01135 flhF 316008..317108 (-) 1101 WP_003220897.1 flagellar biosynthesis protein FlhF -
  PT671_RS01140 flhA 317108..319141 (-) 2034 WP_003220895.1 flagellar biosynthesis protein FlhA -
  PT671_RS01145 flhB 319174..320256 (-) 1083 WP_003220892.1 flagellar biosynthesis protein FlhB -
  PT671_RS01150 fliR 320256..321035 (-) 780 WP_003220890.1 flagellar biosynthetic protein FliR -
  PT671_RS01155 fliQ 321043..321312 (-) 270 WP_003220888.1 flagellar biosynthesis protein FliQ -
  PT671_RS01160 fliP 321327..321992 (-) 666 WP_003220886.1 flagellar type III secretion system pore protein FliP -
  PT671_RS01165 fliZ 321985..322644 (-) 660 WP_003220884.1 flagella biosynthesis regulatory protein FliZ -
  PT671_RS01170 cheY 322659..323021 (-) 363 WP_003220882.1 chemotaxis protein CheY -
  PT671_RS01175 fliY 323047..324186 (-) 1140 WP_003220881.1 flagellar motor switch phosphatase FliY -
  PT671_RS01180 fliM 324176..325174 (-) 999 WP_003220879.1 flagellar motor switch protein FliM -
  PT671_RS01185 fliL 325208..325633 (-) 426 WP_003220878.1 flagellar basal body-associated protein FliL -
  PT671_RS01190 swrD 325630..325845 (-) 216 WP_003220876.1 swarming motility protein SwrD -
  PT671_RS01195 flgG 325885..326679 (-) 795 WP_003220874.1 flagellar basal body rod protein FlgG -
  PT671_RS01200 flgD 326701..327123 (-) 423 WP_003220873.1 flagellar hook assembly protein FlgD -
  PT671_RS01205 - 327120..328583 (-) 1464 WP_003220871.1 flagellar hook-length control protein FliK -
  PT671_RS01210 - 328595..329209 (-) 615 WP_003220869.1 MotE family protein -
  PT671_RS01215 fliJ 329221..329664 (-) 444 WP_003220866.1 flagellar export protein FliJ -
  PT671_RS01220 fliI 329667..330983 (-) 1317 WP_003220864.1 flagellar protein export ATPase FliI -
  PT671_RS01225 fliH 330980..331732 (-) 753 WP_003220862.1 flagellar assembly protein FliH -
  PT671_RS01230 fliG 331725..332741 (-) 1017 WP_003220860.1 flagellar motor switch protein FliG -
  PT671_RS01235 fliF 332754..334364 (-) 1611 WP_079996358.1 flagellar basal-body MS-ring/collar protein FliF -
  PT671_RS01240 fliE 334410..334730 (-) 321 WP_003238544.1 flagellar hook-basal body complex protein FliE -
  PT671_RS01245 flgC 334742..335194 (-) 453 WP_003220854.1 flagellar basal body rod protein FlgC -
  PT671_RS01250 flgB 335194..335583 (-) 390 WP_003220852.1 flagellar basal body rod protein FlgB -
  PT671_RS01255 codY 335964..336743 (-) 780 WP_003220850.1 GTP-sensing pleiotropic transcriptional regulator CodY Regulator
  PT671_RS01260 hslU 336783..338175 (-) 1393 Protein_252 HslU--HslV peptidase ATPase subunit -
  PT671_RS01265 clpQ 338192..338737 (-) 546 WP_003220848.1 ATP-dependent protease subunit ClpQ -
  PT671_RS01270 xerC 338750..339664 (-) 915 WP_003220846.1 tyrosine recombinase XerC -
  PT671_RS01275 trmFO 339732..341039 (-) 1308 WP_003220844.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  PT671_RS01280 topA 341115..343190 (-) 2076 WP_003220843.1 type I DNA topoisomerase -
  PT671_RS01285 dprA 343377..344270 (-) 894 WP_003220839.1 DNA-processing protein DprA Machinery gene
  PT671_RS01290 sucD 344331..345233 (-) 903 WP_003220837.1 succinate--CoA ligase subunit alpha -
  PT671_RS01295 sucC 345262..346419 (-) 1158 WP_003220835.1 ADP-forming succinate--CoA ligase subunit beta -
  PT671_RS01300 - 346593..346874 (-) 282 WP_003220834.1 FlhB-like flagellar biosynthesis protein -
  PT671_RS01305 - 346871..348601 (-) 1731 WP_003220832.1 hypothetical protein -
  PT671_RS01310 rnhB 348630..349397 (-) 768 WP_003220829.1 ribonuclease HII -
  PT671_RS01315 ylqF 349468..350316 (-) 849 WP_003220827.1 ribosome biogenesis GTPase YlqF -
  PT671_RS01320 rplS 350461..350808 (-) 348 WP_003220825.1 50S ribosomal protein L19 -
  PT671_RS01325 trmD 350948..351679 (-) 732 WP_003220823.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  PT671_RS01330 rimM 351676..352200 (-) 525 WP_003220821.1 ribosome maturation factor RimM -
  PT671_RS01335 - 352205..352591 (-) 387 WP_003220819.1 YlqD family protein -
  PT671_RS01340 - 352713..352958 (-) 246 WP_003220817.1 KH domain-containing protein -
  PT671_RS01345 rpsP 352958..353230 (-) 273 WP_003220815.1 30S ribosomal protein S16 -
  PT671_RS01350 ffh 353336..354676 (-) 1341 WP_003220811.1 signal recognition particle protein -
  PT671_RS01355 - 354690..355022 (-) 333 WP_003220809.1 putative DNA-binding protein -
  PT671_RS01360 - 355201..355686 (+) 486 WP_003220807.1 Ig-like domain-containing protein -
  PT671_RS01370 ftsY 356984..357973 (-) 990 WP_003221572.1 signal recognition particle-docking protein FtsY -
  PT671_RS01375 smc 357993..361553 (-) 3561 WP_003221570.1 chromosome segregation protein SMC -
  PT671_RS01380 rncS 361655..362404 (-) 750 WP_013307977.1 ribonuclease III -
  PT671_RS01385 acpP 362544..362777 (-) 234 WP_003154310.1 acyl carrier protein -
  PT671_RS01390 fabG 362861..363601 (-) 741 WP_003221566.1 3-oxoacyl-[acyl-carrier-protein] reductase -
  PT671_RS01395 fabD 363594..364547 (-) 954 WP_003221564.1 ACP S-malonyltransferase -
  PT671_RS01400 plsX 364566..365567 (-) 1002 WP_003221562.1 phosphate acyltransferase PlsX -
  PT671_RS01405 fapR 365581..366147 (-) 567 WP_003221561.1 transcription factor FapR -

Sequence

Protein


Download         Length: 297 a.a.        Molecular weight: 32921.32 Da        Isoelectric Point: 9.9037

>NTDB_id=789121 PT671_RS01285 WP_003220839.1 343377..344270(-) (dprA) [Bacillus spizizenii strain B-354]
MDQAAACLMICRINQLLSPSLLLKWWKADPSMSLTSPVLETVTRGKIKAAALKNKIEEQYPKLQRVLAAYHEQGINPIPI
SSKQYPFWLKTIYDPPAVLFAKGDLTLLSKGRKIGIVGTRNPTAYGIQIVNHLTKELCRKNWVIVSGLASGIDGMSHAAS
IKAKGRTIGVIAGGFQHIYPRENLQLADHMAKHHILLSEHPPETKPQKWHFPMRNRIISGLSEGVIVVQGKEKSGSLITA
YQALEQGREVFAVPGSLFDPYAGGPIKLIQQGAKAIWSAEDIFEELPERNVQYTEPF

Nucleotide


Download         Length: 894 bp        

>NTDB_id=789121 PT671_RS01285 WP_003220839.1 343377..344270(-) (dprA) [Bacillus spizizenii strain B-354]
TTGGATCAGGCCGCTGCCTGTTTAATGATTTGCAGAATCAATCAATTATTATCCCCATCCCTTCTATTAAAATGGTGGAA
AGCTGATCCGTCTATGTCGCTGACTTCACCCGTGTTAGAAACTGTTACTCGAGGTAAAATAAAGGCAGCTGCATTAAAAA
ATAAAATAGAAGAACAATATCCAAAGCTTCAGCGTGTACTTGCTGCTTACCATGAGCAAGGAATTAACCCCATCCCGATT
TCTTCAAAGCAATATCCTTTCTGGCTGAAAACCATTTATGATCCCCCCGCCGTCCTGTTTGCAAAGGGTGATTTGACCCT
TCTTTCTAAAGGGAGGAAAATTGGAATTGTAGGGACAAGAAATCCAACAGCTTATGGGATACAAATTGTCAATCATCTTA
CAAAAGAGCTTTGCCGTAAAAATTGGGTGATTGTCAGCGGACTGGCGTCTGGGATAGACGGAATGTCCCATGCTGCAAGT
ATTAAGGCGAAGGGGCGGACAATCGGCGTCATTGCGGGCGGATTTCAACACATATATCCCCGGGAAAACCTTCAGTTAGC
AGATCATATGGCTAAACACCATATCCTTCTGTCAGAGCACCCACCTGAAACCAAACCCCAAAAATGGCATTTCCCTATGA
GAAACCGTATCATTAGCGGATTAAGTGAAGGCGTTATTGTCGTGCAGGGCAAAGAAAAAAGTGGTTCGCTGATTACTGCA
TATCAAGCACTGGAACAAGGAAGAGAAGTATTTGCCGTACCCGGCTCATTATTTGATCCGTACGCAGGAGGTCCTATAAA
ACTGATCCAGCAGGGGGCTAAAGCCATATGGTCAGCAGAGGATATTTTCGAGGAGCTTCCTGAGAGAAACGTTCAATATA
CGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(71-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0TUA0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

94.276

100

0.943