Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   PUN35_RS00820 Genome accession   NZ_CP117986
Coordinates   164948..167308 (+) Length   786 a.a.
NCBI ID   WP_014480432.1    Uniprot ID   -
Organism   Bacillus sp. N3378-2at     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 159948..172308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN35_RS00800 comN 161279..161575 (+) 297 WP_004398498.1 post-transcriptional regulator ComN -
  PUN35_RS00805 secDF 161614..163827 (+) 2214 WP_014480434.1 protein translocase subunit SecDF -
  PUN35_RS00810 - 163985..164482 (+) 498 WP_014480433.1 cation:proton antiporter regulatory subunit -
  PUN35_RS00815 - 164558..164881 (+) 324 WP_003229740.1 LapA family protein -
  PUN35_RS00820 recJ 164948..167308 (+) 2361 WP_014480432.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  PUN35_RS00825 - 167314..167826 (+) 513 WP_003229745.1 adenine phosphoribosyltransferase -
  PUN35_RS00830 relA 167994..170198 (+) 2205 WP_003229747.1 GTP diphosphokinase -
  PUN35_RS00835 dtd 170211..170651 (+) 441 WP_004398689.1 D-aminoacyl-tRNA deacylase -
  PUN35_RS00840 - 170678..172234 (-) 1557 WP_033881623.1 SH3 domain-containing protein -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 88033.69 Da        Isoelectric Point: 5.1797

>NTDB_id=788866 PUN35_RS00820 WP_014480432.1 164948..167308(+) (recJ) [Bacillus sp. N3378-2at]
MLASKMRWEIQRPDQDKVKSLTEQLHITPLVASLLVKRGFDTAESARLFLHTKDADFYDPFEMKGMKEAADRIKQAISQQ
EKIMIYGDYDADGVTSTSVMLHTLQKLSAQVDFYIPDRFKEGYGPNEQAFRSIKDRGFSLIITVDTGIAAVHEAKVAKEL
GLDVIITDHHEPGPELPDVHAIVHPKQPGCTYPFKELAGVGVAFKLAHALLGELPDELLDLAAIGTIADLVPLHDENRLI
ATLGLERLRRTNRLGLKELIKLSGGDIGEANEETVGFQLAPRLNAVGRIEQADPAVHLLMSEDSFEAEELAAEIDQLNKE
RQKMVSKMTDEAIEMVEQQGLDQTAIVVAKAGWNPGVVGIVASKLVDRFYRPAIVLGIDEEKGIAKGSARSIRGFNLFES
LSECRDILPHFGGHPMAAGMTLKAEDVPDLRSRLNEIADNTLTEEDFIPVQEVDLVCSIEDITVESIAEMNMLSPFGMLN
PKPHVLVENAVLEDVRKIGANKTHVKMTIRNESSQLDCVGFNKGELQEGIVPGSRISIVGEMSINEWNNRKKPQLMIKDA
AVSEWQLFDLRGKRTWEDTVSALPSAKRAIVSFKEDSTTLLQTEDLRREVHVISSKDQAKAFDLDGAYIVLLDPPPSLDM
LARLLEGKAPERIYFIFLNHEDHFLSTFPARDHFKWYYAFLLKRGAFDVKKHGSELAKHKGWSVETINFMTKVFFDLGFV
KIENGVLSVVSGAKKRDLTDSQTYQAKQQLMELDQKLNYSSAEELKEWLNKLMKQDSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=788866 PUN35_RS00820 WP_014480432.1 164948..167308(+) (recJ) [Bacillus sp. N3378-2at]
ATGTTAGCGTCAAAAATGCGATGGGAAATCCAGCGACCAGATCAGGATAAGGTCAAATCACTCACAGAACAATTGCATAT
AACACCTCTTGTTGCGTCCCTGCTTGTAAAAAGAGGCTTTGATACGGCAGAAAGCGCTAGGCTGTTTTTACATACAAAGG
ATGCCGATTTTTATGATCCTTTTGAAATGAAAGGCATGAAAGAAGCGGCAGATCGAATAAAACAGGCGATTTCACAACAA
GAAAAGATTATGATATATGGCGATTATGACGCCGATGGGGTTACAAGTACGTCAGTAATGCTTCACACGCTGCAAAAACT
GTCGGCTCAAGTTGATTTTTATATACCTGACCGTTTTAAAGAAGGATATGGACCTAATGAACAGGCGTTTCGTTCGATTA
AAGATCGGGGCTTCTCTTTGATCATTACGGTTGATACAGGAATTGCCGCTGTGCATGAAGCGAAGGTGGCAAAGGAACTC
GGACTGGATGTCATCATTACTGATCATCATGAACCGGGACCGGAGCTTCCGGATGTCCATGCGATCGTCCACCCGAAGCA
GCCTGGCTGCACTTACCCGTTTAAAGAGCTTGCCGGGGTTGGAGTCGCGTTTAAGCTTGCCCACGCTTTATTGGGTGAAC
TTCCTGATGAACTGCTTGACCTGGCGGCTATCGGCACCATTGCCGATCTAGTCCCGCTGCATGACGAAAACAGATTAATT
GCAACACTAGGACTTGAAAGGCTTCGACGGACAAATCGGCTCGGCTTAAAAGAGCTGATTAAGCTGTCAGGCGGAGACAT
CGGAGAAGCGAACGAAGAAACAGTCGGATTTCAGCTCGCACCAAGGCTGAATGCCGTCGGAAGAATCGAACAGGCGGATC
CCGCTGTTCATTTGCTTATGTCAGAGGATTCGTTTGAAGCGGAAGAGCTTGCCGCAGAAATTGACCAGCTGAACAAAGAG
CGCCAAAAAATGGTCAGCAAAATGACGGATGAAGCGATTGAAATGGTTGAACAGCAAGGGCTCGATCAAACTGCAATTGT
CGTGGCCAAAGCCGGATGGAACCCGGGAGTCGTCGGGATTGTGGCTTCAAAGCTTGTGGACCGCTTCTACCGACCGGCCA
TTGTTCTTGGCATTGATGAAGAAAAGGGAATCGCTAAAGGTTCGGCCCGAAGCATCAGAGGCTTTAATTTATTTGAGAGC
CTTTCTGAATGCAGAGATATTCTTCCTCATTTCGGCGGCCATCCAATGGCTGCGGGGATGACGCTGAAAGCTGAAGATGT
GCCGGATTTGCGAAGCAGGCTGAACGAAATAGCGGACAATACGCTTACGGAAGAGGATTTTATTCCCGTACAGGAAGTCG
ACCTTGTCTGCAGCATCGAGGACATTACAGTTGAAAGCATCGCAGAAATGAACATGCTGTCTCCTTTTGGCATGCTTAAC
CCGAAACCGCACGTTCTCGTAGAAAACGCAGTACTGGAAGATGTCCGCAAGATAGGGGCAAATAAAACTCATGTCAAAAT
GACGATCAGAAACGAGTCGTCCCAGCTTGATTGCGTCGGTTTTAATAAAGGCGAGCTTCAAGAAGGCATCGTACCGGGTT
CAAGAATTTCCATTGTCGGTGAGATGTCAATCAACGAATGGAACAATCGAAAAAAGCCGCAGCTTATGATAAAGGATGCC
GCTGTTTCTGAATGGCAGCTGTTTGATTTAAGGGGAAAACGGACGTGGGAAGACACAGTTTCCGCCCTTCCATCTGCTAA
ACGGGCCATTGTCTCTTTCAAAGAAGACTCAACAACATTACTTCAAACTGAAGACCTTCGCCGAGAAGTGCACGTCATCA
GTTCTAAAGATCAAGCAAAGGCGTTTGACCTCGATGGTGCATATATTGTATTACTGGATCCGCCGCCTTCGCTGGACATG
CTCGCACGTTTACTGGAAGGCAAGGCGCCTGAGCGGATATATTTTATTTTTCTCAATCATGAGGACCACTTTTTATCAAC
TTTTCCCGCAAGAGACCACTTTAAATGGTATTATGCGTTCTTGCTGAAAAGAGGCGCTTTTGACGTGAAAAAACACGGTT
CAGAGCTTGCAAAACATAAAGGCTGGTCCGTTGAAACAATCAATTTCATGACAAAGGTGTTTTTTGATCTCGGTTTTGTT
AAAATAGAGAATGGTGTGCTGTCTGTTGTGAGCGGAGCAAAGAAACGCGACTTAACAGACTCACAAACATATCAGGCGAA
ACAGCAGCTGATGGAACTGGATCAAAAGCTGAATTATTCTTCTGCTGAAGAGCTGAAGGAATGGCTGAATAAGCTGATGA
AACAAGATTCAGAGGCTTATGAAAGTACGAGGAGGACATAA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)

RecJ OB domain (RecJ_OB)

(453-559)

DHHA1 domain (DHHA1)

(315-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

99.491

100

0.995