Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PUB92_RS13500 Genome accession   NZ_CP117919
Coordinates   3020062..3021201 (+) Length   379 a.a.
NCBI ID   WP_017766703.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain PSKL.DM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3015062..3026201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUB92_RS13470 (PUB92_13470) - 3015244..3015624 (-) 381 WP_005302618.1 diacylglycerol kinase -
  PUB92_RS13475 (PUB92_13475) - 3015781..3016842 (+) 1062 WP_005302620.1 3-deoxy-7-phosphoheptulonate synthase -
  PUB92_RS13480 (PUB92_13480) - 3016922..3017707 (-) 786 WP_017783111.1 ATP-binding cassette domain-containing protein -
  PUB92_RS13485 (PUB92_13485) btuC 3017694..3018701 (-) 1008 WP_274181297.1 vitamin B12 ABC transporter permease BtuC -
  PUB92_RS13490 (PUB92_13490) - 3018801..3019532 (-) 732 WP_024942579.1 substrate-binding periplasmic protein -
  PUB92_RS13495 (PUB92_13495) - 3019646..3020002 (+) 357 WP_005302630.1 DMT family protein -
  PUB92_RS13500 (PUB92_13500) pilU 3020062..3021201 (+) 1140 WP_017766703.1 type IVa pilus ATPase TapW Machinery gene
  PUB92_RS13505 (PUB92_13505) glgC 3021484..3022755 (+) 1272 WP_274181298.1 glucose-1-phosphate adenylyltransferase -
  PUB92_RS13510 (PUB92_13510) - 3022890..3023369 (+) 480 WP_065017832.1 WbuC family cupin fold metalloprotein -
  PUB92_RS13515 (PUB92_13515) - 3023489..3023731 (-) 243 WP_219325483.1 FeoC-like transcriptional regulator -
  PUB92_RS13520 (PUB92_13520) feoB 3023728..3026001 (-) 2274 WP_219325482.1 Fe(2+) transporter permease subunit FeoB -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42171.19 Da        Isoelectric Point: 6.2561

>NTDB_id=788349 PUB92_RS13500 WP_017766703.1 3020062..3021201(+) (pilU) [Aeromonas dhakensis strain PSKL.DM]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVASIADAIMDGEQKQQFEKELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPTVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMVKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=788349 PUB92_RS13500 WP_017766703.1 3020062..3021201(+) (pilU) [Aeromonas dhakensis strain PSKL.DM]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGACGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
TGCCAAGTTCAACGGCGGCCTGCGCCCCTTGAGCGAGACGCCGCTCGAGCCCGGCGAGGTGGCCAGCATAGCCGACGCCA
TCATGGACGGCGAGCAGAAGCAGCAGTTTGAGAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGGCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGACTTGAAACTGCCCACAGTGCTGCTCGACACCATCATGGAGAAACGGGGGCTGGTGCTGTTCGTCGGCGGCACCGGTT
CGGGCAAATCCACCTCGCTGGCGGCCCTTATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
CGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTTATCGGCGAAATTCGCGATCGCGAGACCATGGAGCACG
CGCTGGCCTTCTCGGAAACCGGCCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAGCTGCTCAACGATCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTGAAAACCACTGATGGCGGCCGCCGGGCTGCGGTGGAGATCATGCTGGGCACCCACACCATCCGCGACATGGTCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGGTGATGGAGAAGTCCAAGGCCCTCGGCATGGTCACCTTCGACAGTGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATCGACGAGGAGGAGGCAGTGAAGAACGCCGATTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTATGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTGGAGCCCACCAAGGACG
AAAAGGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.867

98.945

0.533
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.793

93.14

0.38
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Legionella pneumophila strain ERS1305867

41.317

88.127

0.364
  pilT Legionella pneumophila strain Lp02

41.317

88.127

0.364
  pilT Pseudomonas stutzeri DSM 10701

39.826

90.765

0.361