Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   Q5419_RS25485 Genome accession   NZ_CP130752
Coordinates   5462284..5462709 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain AU10241 isolate AU10241_L13_Resistant     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5457284..5467709
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5419_RS25470 (Q5419_25470) pilX 5457848..5458435 (+) 588 WP_003102605.1 type 4a pilus minor pilin PilX -
  Q5419_RS25475 (Q5419_25475) pilY1 5458447..5461938 (+) 3492 WP_003102607.1 type 4a pilus biogenesis protein PilY1 -
  Q5419_RS25480 (Q5419_25480) pilY2 5461940..5462287 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  Q5419_RS25485 (Q5419_25485) comF 5462284..5462709 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  Q5419_RS25490 (Q5419_25490) ispH 5462756..5463700 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  Q5419_RS25495 (Q5419_25495) fkpB 5463786..5464226 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  Q5419_RS25500 (Q5419_25500) lspA 5464219..5464728 (-) 510 WP_003102615.1 signal peptidase II -
  Q5419_RS25505 (Q5419_25505) ileS 5464721..5467552 (-) 2832 WP_003102617.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=787878 Q5419_RS25485 WP_003094721.1 5462284..5462709(+) (comF) [Pseudomonas aeruginosa strain AU10241 isolate AU10241_L13_Resistant]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=787878 Q5419_RS25485 WP_003094721.1 5462284..5462709(+) (comF) [Pseudomonas aeruginosa strain AU10241 isolate AU10241_L13_Resistant]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383