Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   PQ478_RS04130 Genome accession   NZ_CP117835
Coordinates   802680..805049 (+) Length   789 a.a.
NCBI ID   WP_289235912.1    Uniprot ID   -
Organism   Alkalihalophilus pseudofirmus strain DSM 8715     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 797680..810049
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PQ478_RS04100 (PQ478_04100) - 798019..798504 (+) 486 WP_289235910.1 COG2426 family protein -
  PQ478_RS04105 (PQ478_04105) - 798541..798816 (+) 276 WP_022626512.1 post-transcriptional regulator -
  PQ478_RS04110 (PQ478_04110) secD 798970..800274 (+) 1305 WP_289235911.1 protein translocase subunit SecD -
  PQ478_RS04115 (PQ478_04115) secF 800264..801208 (+) 945 WP_022626514.1 protein translocase subunit SecF -
  PQ478_RS04120 (PQ478_04120) - 801333..802232 (+) 900 WP_012957767.1 cation diffusion facilitator family transporter -
  PQ478_RS04125 (PQ478_04125) - 802263..802601 (+) 339 WP_012957768.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  PQ478_RS04130 (PQ478_04130) recJ 802680..805049 (+) 2370 WP_289235912.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  PQ478_RS04135 (PQ478_04135) - 805052..805567 (+) 516 WP_012957770.1 adenine phosphoribosyltransferase -
  PQ478_RS04140 (PQ478_04140) - 805780..807966 (+) 2187 WP_075683110.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  PQ478_RS04145 (PQ478_04145) dtd 807991..808431 (+) 441 WP_012957772.1 D-aminoacyl-tRNA deacylase -
  PQ478_RS04150 (PQ478_04150) - 808504..808710 (+) 207 WP_022626518.1 hypothetical protein -

Sequence

Protein


Download         Length: 789 a.a.        Molecular weight: 88587.03 Da        Isoelectric Point: 5.0785

>NTDB_id=787840 PQ478_RS04130 WP_289235912.1 802680..805049(+) (recJ) [Alkalihalophilus pseudofirmus strain DSM 8715]
MLRPKARWKIQEQDEHKAGRLVSELGVDELVAQLLVNRGIETPEAARTFLYTKEMTYHDPYLLKGMDKAVARIKEAIANQ
EKILIFGDYDADGVSSTSVMIYTLNELGAVFDYYIPNRFTEGYGPNEPALRRAKEEGYSLVVTVDTGISAVHEANVAKEI
DLDFIVTDHHEAPPVLPEAYAIVNPKQPDCTYPFKGLAGVGVAFKVSQALLGRIPKELLDIVVIGTIADLVPLIDENRLM
AKEGLKALESTSKPGLRALMKVCGIGQDALTGDHVGFGIGPRINAAGRLDSADPAVELMITNDSNEAEHLAAEIDSFNKQ
RQSIVNTITEEAVEVVESKYPPDENDVLIIAGAGWNAGVIGIVASRLVERFYRPTIVLSIDEEKGLAKGSARSIEGFDMF
KELSKSRDILPHFGGHPMAAGLTMKESDLNLLRERLNNQAKEVLTEEDFKPLTKIDLSTTVDRISLTVIKQLEALAPFGV
NNPTPKVMLEGVHIDQMRRIGSESNHLKMSFVQNGASLDGIGFHLGHLHDEISQTAKLSAIGTLSINEWNGHVKPQMMLE
DLAVKEWQLFDWRSIKQKRLEDRLMEIPIDDRVLIFFKESTKEELGLQLDDLRKASDLTEMSGGFEGKYVVLLDVPNTRE
ELSLLFKEQGTPSRIYTIFHQQDDSFFTTNPNRDQFKWFYAFLLKRKTFDVKKHGTDLAKHKGWSRHTIDFMSQVFLELG
FVTIDNGVLSLSDAPEKKALDASITYQRKQEQAQIENQFVYSSYEELKQWFNRVIPRNEIDERQIKETV

Nucleotide


Download         Length: 2370 bp        

>NTDB_id=787840 PQ478_RS04130 WP_289235912.1 802680..805049(+) (recJ) [Alkalihalophilus pseudofirmus strain DSM 8715]
ATGTTAAGACCTAAGGCAAGATGGAAAATCCAAGAACAAGATGAACATAAAGCAGGTAGGCTTGTAAGCGAGCTGGGCGT
TGATGAACTTGTTGCCCAATTATTAGTTAATCGAGGCATAGAGACACCAGAAGCTGCGAGAACTTTTTTATATACAAAGG
AAATGACGTACCATGATCCCTATTTGTTAAAGGGTATGGACAAGGCTGTGGCACGAATTAAAGAAGCCATTGCAAACCAA
GAGAAGATCTTAATATTTGGTGACTATGATGCGGACGGTGTAAGCAGCACCTCTGTTATGATTTACACGTTAAATGAACT
TGGTGCTGTGTTTGATTATTATATTCCTAATCGGTTCACAGAAGGCTACGGGCCTAATGAGCCGGCTTTGAGGCGTGCGA
AAGAAGAGGGGTATTCCTTAGTCGTAACAGTAGATACAGGGATTTCTGCTGTTCACGAGGCGAATGTCGCAAAAGAGATT
GATCTTGATTTTATTGTGACCGACCATCATGAGGCCCCTCCAGTTTTGCCAGAGGCATATGCTATCGTAAACCCGAAGCA
GCCAGATTGTACGTATCCTTTTAAAGGATTAGCTGGTGTAGGGGTTGCGTTCAAAGTGTCACAAGCACTCCTCGGCAGAA
TTCCTAAAGAGCTGCTTGATATTGTTGTTATCGGAACGATTGCTGATCTAGTACCGCTGATTGATGAGAATCGTTTAATG
GCTAAAGAAGGACTGAAGGCCCTAGAATCAACATCGAAGCCAGGGTTGCGTGCTTTGATGAAAGTGTGTGGAATTGGTCA
GGATGCCTTAACGGGCGATCATGTCGGCTTTGGAATTGGACCGCGGATCAATGCTGCAGGAAGACTTGATTCAGCTGATC
CGGCAGTAGAGTTGATGATTACGAATGATTCAAATGAAGCGGAACACCTAGCTGCTGAAATTGATTCTTTTAATAAACAA
CGTCAATCGATTGTTAATACGATTACTGAAGAAGCAGTCGAGGTCGTAGAATCTAAGTATCCTCCAGATGAGAATGATGT
GTTGATCATTGCAGGAGCCGGCTGGAATGCAGGTGTTATTGGGATTGTTGCCTCACGCCTTGTTGAGCGTTTCTATCGTC
CGACAATCGTATTAAGTATTGACGAAGAAAAAGGGTTAGCCAAAGGTTCAGCGAGGAGTATTGAAGGCTTTGATATGTTT
AAAGAATTATCGAAAAGCCGAGATATCCTGCCTCATTTTGGAGGACATCCAATGGCGGCTGGATTGACAATGAAAGAATC
TGATCTTAATTTACTGCGTGAACGTTTAAATAATCAGGCGAAGGAAGTTCTAACTGAGGAAGACTTTAAGCCGCTTACGA
AGATCGATTTATCCACAACGGTAGACCGCATCTCCTTAACGGTGATTAAGCAGTTAGAAGCTCTAGCTCCTTTTGGAGTG
AATAATCCAACACCAAAAGTGATGCTTGAAGGAGTTCATATCGATCAGATGCGCCGTATTGGCAGTGAGTCTAATCATCT
AAAAATGTCTTTTGTTCAAAACGGAGCGAGTCTTGATGGGATTGGCTTTCATTTAGGGCATTTACATGATGAAATCAGTC
AGACGGCAAAGCTCTCTGCGATTGGAACTCTTTCCATCAATGAATGGAATGGACATGTAAAACCGCAGATGATGCTTGAA
GATCTTGCAGTTAAGGAATGGCAGCTTTTTGACTGGCGCAGTATTAAACAAAAGAGGTTAGAAGATCGATTGATGGAAAT
TCCGATTGATGACCGTGTACTTATTTTCTTCAAAGAATCCACGAAAGAAGAGCTCGGACTCCAGCTGGATGACTTGAGAA
AAGCGAGTGATCTGACGGAGATGAGCGGAGGCTTTGAAGGGAAGTATGTGGTATTATTAGATGTGCCTAATACTCGAGAA
GAGCTTTCTCTTTTATTTAAAGAACAAGGAACACCTAGCAGGATTTATACTATTTTTCATCAACAGGACGATTCGTTTTT
TACGACAAATCCAAATCGTGATCAATTTAAATGGTTCTATGCTTTTTTATTAAAGCGTAAAACATTCGATGTGAAAAAAC
ATGGAACAGACCTTGCTAAGCATAAAGGCTGGTCTAGACATACGATCGATTTTATGTCCCAGGTGTTTTTAGAGCTCGGT
TTTGTTACAATAGACAATGGCGTTCTATCTCTAAGTGATGCACCAGAGAAAAAAGCGCTCGATGCGTCAATTACGTATCA
ACGAAAACAAGAGCAAGCACAAATAGAAAATCAATTCGTTTATTCTTCTTATGAGGAATTAAAGCAATGGTTCAATCGCG
TGATTCCCCGAAATGAAATAGATGAACGACAAATCAAGGAGACGGTGTAA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-771)

DHHA1 domain (DHHA1)

(315-438)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.746

99.24

0.523